[1]韩 磊,张文卿,孙 寰,等.骨髓间充质干细胞不同途径输注对阿霉素肾病大鼠AQP1、AQP2表达的影响 [J].介入放射学杂志,2017,(11):1015-1019.
 HAN Lei,ZHANG Wenqing,SUN Huan,et al.Influence of infusion of mesenchymal stem cells by different routes on the expression of AQP1 and AQP2 in rats with adriamycin- induced nephropathy[J].journal interventional radiology,2017,(11):1015-1019.
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骨髓间充质干细胞不同途径输注对阿霉素肾病大鼠AQP1、AQP2表达的影响


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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2017年11期
页码:
1015-1019
栏目:
实验研究
出版日期:
2017-11-25

文章信息/Info

Title:
Influence of infusion of mesenchymal stem cells by different routes on the expression of AQP1 and AQP2 in rats with adriamycin- induced nephropathy
作者:
韩 磊 张文卿 孙 寰 陆发承 简远熙 邢艺苑 王家平
Author(s):
HAN Lei ZHANG Wenqing SUN Huan LU Facheng JIAN Yuanxi XIN Yiyuan WANG Jiaping
Department of Radiology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province 650101, China
关键词:
【关键词】 骨髓间充质干细胞 移植途径 阿霉素肾病 AQP1 AQP2
文献标志码:
A
摘要:
【摘要】 目的 评估骨髓间充质干细胞(MSC)分别通过肾动脉和尾静脉移植方式对大鼠慢性肾病(CKD)模型肾损伤修复的疗效,比较不同移植方式对CKD大鼠水通道蛋白(AQP)1、AQP2表达差异的影响。方法 实验取50只大鼠,2只制备骨髓MSC;36只经尾静脉输注阿霉素构建CKD模型,随机分为阿霉素CKD(A- C)组(n=12)、骨髓MSC经肾动脉输注(M- A)组(n=12)、骨髓MSC经尾静脉输注(M- V)组(n=12);另12只为正常对照(N)组。末次骨髓MSC移植隔1周后检测大鼠24 h尿量、24 h尿蛋白定量、血清钠及血清白蛋白,免疫组化检测肾组织AQP1、AQP2表达。结果 与A- C组相比,M- V组、M- A 组大鼠血清白蛋白及 24 h尿量均增高(P<0.05),24 h尿蛋白及血清钠水平均降低(P<0.05),M- A组24 h尿蛋白较M- V组降低更明显(P<0.05)。M- V组、M- A组大鼠肾脏AQP1、AQP2表达均较A- C组降低(P<0.05),M- V组、M- A组间差异无统计学意义(P<0.05)。结论 骨髓MSC移植可增加血清白蛋白,降低尿蛋白、血清钠和肾实质细胞内AQP1、AQP2表达,对修复阿霉素CKD大鼠肾损伤有作用。在一定时间内肾动脉途径移植效果优于外周静脉途径,但两种移植途径的效果差异与AQP1、AQP2表达无明显关联。

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备注/Memo

备注/Memo:
(收稿日期:2017-03-21)
(本文编辑:边 佶)
更新日期/Last Update: 2017-11-16