[1]杨 扬,王家平,万珊杉,等.肾动脉移植骨髓间充质干细胞对阿霉素肾病大鼠肾纤维化的影响[J].介入放射学杂志,2020,29(08):802-806.
 YANG Yang,WANG Jiaping,WAN Shanshan,et al.The influence of renal arterial transplantation of bone marrow mesenchymal stem cells on renal fibrosis in experimental rats with adriamycin-induced nephropathy[J].journal interventional radiology,2020,29(08):802-806.
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肾动脉移植骨髓间充质干细胞对阿霉素肾病大鼠肾纤维化的影响()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
29
期数:
2020年08
页码:
802-806
栏目:
实验研究
出版日期:
2020-08-25

文章信息/Info

Title:
The influence of renal arterial transplantation of bone marrow mesenchymal stem cells on renal fibrosis in experimental rats with adriamycin-induced nephropathy
作者:
杨 扬 王家平 万珊杉 李天祎 杨素萍
Author(s):
YANG YangWANG JiapingWAN ShanshanLI TianyiYANG Suping.
Department of Radiology,Second Affiliated Hospital of Kunming Medical University,Kunming,Yunnan Province 650101,China
关键词:
【关键词】 骨髓间充质干细胞 慢性肾脏病 肾脏纤维化 肾动脉 移植治疗
文献标志码:
A
摘要:
【摘要】 目的 分析经肾动脉移植骨髓间充质干细胞(BMSC)对阿霉素慢性肾脏病(CKD)大鼠的治疗作用,评估不同移植方式对肾脏纤维化的影响。方法 实验动物为50只雄性Sprague-Dawley大鼠,其中2只4周龄大鼠用于体外分离培养BMSC;36只16周龄大鼠经尾静脉注射阿霉素构建CKD动物模型,造模成功后随机分为模型组(CKD组)、经尾静脉移植BMSC组(V-M组)和经肾动脉移植BMSC组(A-M组),每组各12只;12只健康大鼠作为正常对照组(N组),经尾静脉输注等量0.9%氯化钠溶液。移植BMSC后第7、14天检测各组大鼠血肌酐、血尿素氮、24 h尿蛋白水平;制作肾脏病理切片行HE染色观察肾脏结构病理改变,Masson三色染色评估肾脏纤维化程度。结果 CKD组、V-M组、A-M组在各观察点血肌酐、血尿素氮、24 h尿蛋白水平均显著高于N组(P<0.01)。移植后第7天,V-M组、A-M组24 h尿蛋白水平均低于CKD组(P<0.05),且A-M组低于V-M组(P<0.05);A-M组血肌酐、血尿素氮显著低于CKD组(P<0.01)。移植后第14天,V-M组、A-M组24 h尿蛋白水平均低于CKD组,但差异无统计学意义(P>0.05);A-M组血肌酐、血尿素氮均低于V-M组、CKD组(P<0.05)。CKD组、V-M组、A-M组肾组织HE染色呈慢性肾病改变,Masson三色染色示肾间质及肾小囊周围不同程度纤维化,但A-M组纤维化程度较V-M组、CKD组有所改善。结论 经肾动脉移植BMSC可抑制或延缓CKD大鼠肾脏纤维化,并发挥积极的治疗作用。

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备注/Memo

备注/Memo:
(收稿日期:2019- 09- 22)
(本文编辑:边 佶)
更新日期/Last Update: 2020-08-17