[1]杨 扬,王家平,白彝华,等.不同剂量骨髓间充质干细胞对阿霉素肾病大鼠肾纤维化形成的影响[J].介入放射学杂志,2020,29(12):1222-1226.
 YANG Yang,WANG Jiaping,BAI Yihua,et al.Effect of different doses of bone marrow mesenchymal stem cells on renal fibrosis in experimental rats with adriamycin-induced nephropathy[J].journal interventional radiology,2020,29(12):1222-1226.
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不同剂量骨髓间充质干细胞对阿霉素肾病大鼠肾纤维化形成的影响()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
29
期数:
2020年12
页码:
1222-1226
栏目:
实验研究
出版日期:
2020-12-25

文章信息/Info

Title:
Effect of different doses of bone marrow mesenchymal stem cells on renal fibrosis in experimental rats with adriamycin-induced nephropathy
作者:
杨 扬 王家平 白彝华 李天祎 李嘉琦 张 娅
Author(s):
YANG Yang WANG Jiaping BAI Yihua LI Tianyi LI Jiaqi ZHANG Ya.
Department of Radiology, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan Province 650101, China
关键词:
【关键词】 骨髓间充质干细胞 肾动脉 肾纤维化 转化生长因子-β1
文献标志码:
A
摘要:
【摘要】 目的 探讨不同剂量骨髓间充质干细胞(BMSC)对阿霉素慢性肾病大鼠肾纤维化形成的影响及其作用机制。方法 SD大鼠尾静脉注射阿霉素造模,首次剂量3 mg/kg,2周后相同剂量再次注射。造模成功后将大鼠随机分为M组(模型组,肾动脉注射磷酸缓冲液),A组、B组、C组(肾动脉分别移植1×106个/mL、2×106个/mL、3×106个/mL BMSC,细胞悬液均为0.5 mL),每组各9只。另取9只正常大鼠作为对照组(N组,尾静脉输注等量0.9%氯化钠溶液)。术后第4周测定各组大鼠体重,Masson染色评估肾纤维化程度,过碘酸六胺银(PASM)染色观察肾小球和肾小管基膜改变,免疫组化染色分析转化生长因子(TGF)-β1、纤维连接蛋白(FN)表达。结果 BMSC移植4周后,A、B、C组大鼠一般情况均较M组改善,体重改变最明显,体重增长变化为C组>B组>A组,但C组与B组差异无统计学意义(P>0.05)。Masson染色显示M组表现为典型的肾小球和肾小管纤维化改变,肾组织大面积蓝染,A、B、C组虽也有不同程度肾纤维化改变,但程度和范围均较M组减少(P<0.05)。PASM染色显示M组肾小球和肾小管基膜明显增厚,A、B、C组肾小球和肾小管基膜增厚程度下降(P<0.05)。免疫组化染色显示A、B、C组肾组织不同部位TGF-β1、FN表达与M组比较均下降,尤其是C组下降最明显,仅在部分肾小球和肾小管上皮细胞见少量弱阳性表达。结论 BMSC移植治疗可延缓阿霉素慢性肾病大鼠早期肾纤维化形成,且该作用随细胞移植数量增加而增强。其机制可能是通过下调TGF-β1表达抑制细胞外基质过度沉积实现的。

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备注/Memo

备注/Memo:
(收稿日期:2020- 02- 02)
(本文编辑:边 佶)
更新日期/Last Update: 2020-12-16