[1]卢勤,牛焕章,朱光宇,等.经导管动脉输送转铁蛋白增强p５３基因转染效率的研究[J].介入放射学杂志,2007,(02):99.
　LU Qin,NIU Huan-zhang,ZHU Guang-yu,et al.Investigation of transfection efficacy with transcatheter arterial transporting transferrin to enhance p５３gene[J].journal interventional radiology,2007,(02):99.
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经导管动脉输送转铁蛋白增强 p５３ 基因转染效率的研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2007年02期
页码:
99
栏目:
实验研究
出版日期:
2007-02-15

文章信息/Info

Title:
Investigation of transfection efficacy with transcatheter arterial transporting transferrin to enhance p５３gene
作者:
卢勤; 牛焕章; 朱光宇; 等
２１０００９南京东南大学附属中大医院放射科（卢勤、牛焕章、朱光宇、安艳丽、滕皋军）；东南大学基础医学院生物化学系（邱定红）
Author(s):
LU Qin; NIU Huan-zhang; ZHU Guang-yu; AN Yan-li; QIU Ding-hong; TENG Gao-jun．
Radiologic Department，Zhongda Hospital，Southeast University，Nanjing２１０００９，China
关键词:
转铁蛋白; 介入; p５３基因; 转染效率; 肝癌细胞
分类号:
R７３５.７
文献标志码:
A
摘要:
目的研究转铁蛋白（Tf）对p５３基因转染效率的影响，并探讨介入技术与Tf联合应用对基因治疗肝癌的双重靶向作用。方法应用pCMV-myc-p５３质粒与阳离子脂质体LipofectAMINE复合物，转染３种肝癌细胞株LM６、Hep３B、YY以及正常肝细胞株L０２，以不同浓度Tf（０、１０、２５、５０及１００μg）介导转染，蛋白印迹法检测各细胞株中p５３表达情况，并对Tf影响p５３转染效率的关系进行分析。再建立兔VX２肝癌动物模型，经介入导管输注Tf-质粒-脂质体复合物，提取肿瘤组织蛋白，蛋白印迹法检测P５３表达。结果４种细胞应用p５３-脂质体复合物以及不同浓度Tf转染后４８h的蛋白印迹法检测，发现Tf在１０～１００μg可明显增强p５３-脂质体的转染效率，且转染效率随Tf浓度增加而增强。动物模型组织提取蛋白检测结果，显示Tf明显增强P５３蛋白表达。结论Tf能增强脂质体-基因转染，介入技术与Tf联合双重靶向对肝癌的基因治疗具有相当的应用前景。

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备注/Memo

备注/Memo:
收稿日期：２００６-１０-１２
更新日期/Last Update: 2007-02-15