[1]储玉山,朱小庆,张玉宇,等.索拉非尼联合奥沙利铂对肝癌HepG2细胞的抑制作用[J].介入放射学杂志,2011,(10):810.
 CHU Yu-shan,ZHU Xiao-qing,ZHANG Yu-yu,et al. The inhibitory effect of sorafenib together with oxaliplatin on the growth of human hepatic carcinoma cell line HepG2 in vitro[J].journal interventional radiology,2011,(10):810.
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索拉非尼联合奥沙利铂对肝癌HepG2细胞的抑制作用()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2011年10期
页码:
810
栏目:
实验研究
出版日期:
2011-10-25

文章信息/Info

Title:

The inhibitory effect of sorafenib together with oxaliplatin on the growth of human hepatic carcinoma cell line HepG2 in vitro

作者:
储玉山 朱小庆 张玉宇 陆小华 曹建民
江苏省南通大学附属医院介入放射科
Author(s):

CHU Yu-shan ZHU Xiao-qing ZHANG Yu-yu LU Xiao-hua CAO Jian-min.

Department of Interventional Radiology, Affiliated Hospital of Nantong University, Nantong, Jiangsu Province 226001, China
关键词:
肝癌 索拉非尼 奥沙利铂 联合用药 协同作用
分类号:
R735.7
文献标志码:
A
摘要:
目的   探讨索拉非尼联合奥沙利铂(LOHP)对肝癌细胞HepG2的抑制作用及其可能的分子机制。方法 索拉非尼和LOHP单独及联合给药后以CCK8法测定HepG2细胞的增殖,用流式细胞仪检测细胞周期及凋亡,用Western blot观察ERK及pERK蛋白的表达。结果 索拉非尼及LOHP单药对HepG2均有抑制作用,两药联合具有协同或相加作用(P < 0.05)。索拉非尼及LOHP分别主要使细胞阻滞于G1及S期。两药联用后同时阻滞于G1及S期。联合组细胞凋亡率明显比各单药组高(P < 0.05)。单用及联合用药对HepG2细胞ERK表达无明显影响,索拉非尼及联合用药减少pERK的表达。结论 索拉非尼和LOHP对肝癌HepG2细胞有增殖抑制及促凋亡作用,两药联合表现为协同或相加作用,其机制可能与细胞周期的双重阻滞及细胞增殖通路Raf/MEK/ERK的抑制有关。

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备注/Memo

备注/Memo:
收稿日期:20110604
更新日期/Last Update: 2011-10-25