[1]曾江正,蔡兴锐,黄 芬,等.体外不完全射频消融后肝癌细胞上皮-间质转化相关长链非编码RNA差异表达 [J].介入放射学杂志,2017,(09):823-829.
　ZENG Jiangzheng,CAI Xingrui,HUANG Fen,et al.Differential expression profile of EMT- related LncRNAs in hepatocellular carcinoma cells after incomplete radiofrequency ablation in vitro [J].journal interventional radiology,2017,(09):823-829.
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体外不完全射频消融后肝癌细胞上皮-间质转化相关长链非编码RNA差异表达 


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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2017年09期
页码:
823-829
栏目:
实验研究
出版日期:
2017-09-25

文章信息/Info

Title:
Differential expression profile of EMT- related LncRNAs in hepatocellular carcinoma cells after incomplete radiofrequency ablation in vitro
作者:
曾江正;  蔡兴锐;  黄 芬;  雷俊华;  何志惠;  孙华茂;  卢彦达
Author(s):
ZENG Jiangzheng;  CAI Xingrui;  HUANG Fen;  LEI Junhua;  HE Zhihui;  SUN Huamao;  LU Yanda
Department of Medical Oncology， First Affiliated Hospital of Hainan Medical College， Cancer Institute of Hainan Medical College， Haikou， Hainan Province 570102， China
关键词:
【关键词】 长链非编码RNA;  射频消融术;  肝癌;  上皮-间质转化
文献标志码:
A
摘要:
【摘要】 目的 研究上皮-间质转化（EMT）相关长链非编码RNA（LncRNA）在不完全射频消融（RFA）肝细胞癌（HCC）病灶的差异表达谱变化。方法 采用Huh7细胞47℃水浴加热，获取体外模拟的不完全RFA HCC细胞。镜下及免疫印迹检测Huh7- H细胞EMT改变，Transwell检测细胞迁移与侵袭能力，CCK- 8检测细胞增殖能力。采用LncPathTM Human EMT Array芯片分析Huh7- H和Huh7差异表达的EMT相关LncRNA，RT- PCR验证。结果 镜下显示Huh7- H呈现EMT形态学改变。免疫印迹检测显示Huh7- H细胞上皮表型标志分子E- cadherin表达降低，间质表型标志分子N- cadherin、vimentin表达增加。Transwell迁移及侵袭实验显示，Huh7- H、Huh7穿膜细胞数分别为（138.0±11.8）和（61.0±5.2）、（82.7±39.4）和（33.3±7.8），Huh7- H细胞迁移及侵袭能力明显增强（P＜0.05）。CCK- 8检测显示Huh7- H细胞增殖能力明显增强（P＜0.05）。LncPathTM Human EMT Array芯片筛选出3个差异表达LncRNA（P≤0.05，变化倍数≥1.5），即2条LncRNA（FUNDC2P4、RPL27P7）下调，1条LncRNA（MTND4LP14）上调。RT- PCR验证HUH7- H组FUNDC2P4、RPL27P7、MTND4LP14基因表达丰度分别是HUH- 7组的0.137、0.869、1.037倍。临床标本验证LncRNA FUNDC2P4在癌旁组织表达高于HCC组织，HCC组织高于RFA术后残余HCC组织。结论 筛选出不完全RFA后HCC细胞低表达的EMT相关LncRNA FUNDC2P4，为深入探讨该基因功能及分子机制提供了基础。

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备注/Memo

备注/Memo:
（收稿日期：２０１7-０4-18）
（本文编辑：边 佶）
更新日期/Last Update: 2017-09-12