[1]王以浪,王亚非,张 亮,等. 肝细胞癌患者TACE术前后血清miR- 21表达变化及临床意义[J].介入放射学杂志,2014,(05):406-410.
　WANG Yi- lang,WANG Ya- fei,ZHANG Liang,et al. The changes of serum miR- 21 expression level in patients with HCC before and after TACE and its clinical significance[J].journal interventional radiology,2014,(05):406-410.
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 肝细胞癌患者TACE术前后血清miR- 21表达变化及临床意义()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2014年05期
页码:
406-410
栏目:
肿瘤介入
出版日期:
2014-05-28

文章信息/Info

Title:
 The changes of serum miR- 21 expression level in patients with HCC before and after TACE and its clinical significance
作者:
 王以浪;  王亚非;  张 亮;  缪亚军;  陈 卓;  周 陈;  印 滇;  丁文彬
Author(s):
 WANG Yi- lang;  WANG Ya- fei;  ZHANG Liang;  MIAO Ya- jun;  CHEN Zhuo;  ZHOU Chen;  YIN Dian;  DING Wen- bin.
 The First People’s Hospital of Nantong， Natong， Jiangsu Province 226001， China
关键词:
 ; 【关键词】 肝细胞癌;  经导管动脉化疗栓塞术;  miRNA- 21;  血清
文献标志码:
A
摘要:
 【摘要】 目的 探讨HCC患者经皮肝动脉化疗栓塞（TACE）术前、后血清miR- 21表达变化及临床意义。方法 以反转录定量PCR（RT- PCR）法检测HCC患者TACE术前、后及正常者血清miR- 21水平，以酶联免疫吸附法（ELISA）检测血清AFP水平。结果 HCC患者血清miR- 21水平为正常人的（12.9 ± 3.5）倍（t = 19.430 7，P < 0.01），TACE术后1个月为正常参考值的（7.2 ± 1.7）倍，较术前显著降低（t = 9.493 7，P < 0.01）。血清 miR- 21水平与肿瘤大小、癌栓及HBV感染相关。TACE术后1个月血清miR- 21水平在部分缓解、稳定和进展组中分别为正常人的（4.0 ± 0.3）、（6.0 ± 1.5）和（8.6 ± 1.5）倍，各组间差异有统计学意义（F = 38.168，P = 0.000）。miR- 21诊断HCC的ROC- AUC值为0.910 ± 0.041，显著高于AFP的0.860 ± 0.037（t = 6.304 2，P < 0.01）。miR- 21检测HCC的特异度（88.1%）显著高于AFP（69.0%，χ2 = 4.525 3，P = 0.033）。结论 TACE术后miR- 21水平明显降低，能较好预测TACE术疗效，是HCC的潜在分子标记物。

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 （收稿日期：２０１3-11-13）
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