[1]陈晟,邓钢,牛焕章,等.兔VX２肝种植肿瘤模型制作的完善及综合影像学评价[J].介入放射学杂志,2007,(01):48.
　CHEN Sheng,DENG Gang,NIU Huan-zhang,et al.Consummation in making rabbit model bearing VX２liver tumor and multiple imaging evaluation[J].journal interventional radiology,2007,(01):48.
点击复制

兔 VX２ 肝种植肿瘤模型制作的完善及综合影像学评价()

PDF下载中关闭

分享到：

《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2007年01期
页码:
48
栏目:
实验研究
出版日期:
2007-01-15

文章信息/Info

Title:
Consummation in making rabbit model bearing VX２liver tumor and multiple imaging evaluation
作者:
陈晟; 邓钢; 牛焕章; 何仕诚; 方文; 汪盛齐; 马占龙; 郭金和; 李国昭; 滕皋军
２１０００９南京东南大学附属中大医院放射科
Author(s):
CHEN Sheng; DENG Gang; NIU Huan-zhang; HE Shi-cheng; FANG Wen; WANG Sheng-qi; MA Zhan-long; GUO jin-he; LI Guo-zhao; TENG Gao-jun．
Department of Radiology，Zhong-Da Hospital，Southeast University，Nanjing２１０００９，China
关键词:
肝肿瘤; VX２; 动物模型; 影像学评估
分类号:
R７３.３５４
文献标志码:
A
摘要:
目的进一步完善兔VX２肝种植肿瘤模型的制作，比较种植肿瘤的CT、MRI及DSA影像表现，评价各影像检查对瘤体显示的优越性。方法实验动物为新西兰大白兔（前期实验１２只，后期实验２４只），采用动物后肢皮下注射接种传代保存瘤种。模型前期制作采用包埋法接种，后期采用针头注入法接种于兔肝左叶，并于术前１d及术后１、３d、１、２周检查肝肾功能的动态变化情况。接种２周后行CT、MR成像，随后行经股动脉肝动脉超选择DSA及纳米磁性粒子介入治疗。结果前期实验肿瘤种植成功率６６．７％，后期实验种植成功率１００％。术后ALT、AST有一过性增高，无统计学意义；BUN、Cr无明显变化。术后CT平扫肿瘤为略低密度病灶，强化不明显，境界清晰；MR平扫呈长T１、长T２信号；EPI序列T２WI呈略高信号影；DWI成像见高亮信号影，显示清晰；介入术中DSA造影可见肝左叶孤立的丰富血管肿瘤，供血动脉增粗，经超选择注入纳米磁性粒子，肿瘤内密度明显增高。结论兔VX２肝种植肿瘤模型是介入治疗实验研究理想的动物模型。VX２瘤粒针头注入法较包埋法成功率更高，其建立过程对兔肝肾功能无明显影响。CT平扫增强，MR平扫，EPI序列及DWI成像对于肿瘤的影像评价互有优势，EPI及DWI成像可更灵敏地显示肿瘤，DSA可清晰地显示肿瘤的供血及肝内有无转移。

参考文献/References:

［１］贾雨辰，程红岩，陈栋，等．原发性肝癌的介入治疗［J］．肝胆外科杂志，１９９８，６：３１６-３１８．
［２］Camma C，Schepis F，Orlando A，et al．Transcatheter chemo-embolization for unresectable hepatocellular carcinoma：met-analysis of randomized controlled trials［J］．Radiology，２００２，２２４：５４-５７．
［３］Livraghi T．Radiofrequency ablation，PEIT，and TACE for hepatocelluar carcinoam［J］．Hepatobiliary Pancreat Surg，２００３，１０：６７-７６．
［４］Shope RE，Hurst EW．Infectious papillomatosis of rabbits：with a note on the histopathology［J］．J Exp Med，１９３３，５８：６０７-６２４．
［５］Rous P，Kidd JG，Smith WE．Experiments on the cause of the rabbit carcinomas derived from virus-induced papillomas．II．Lossby the VX２carcinoma of the power to immunize hosts against the papilloma virus［J］．J Exp Med，１９５２，９６：１５９-１７４．
［６］邵国良，周康荣，王建华，等．介入治疗实验研究中兔VX２肝癌模型制作的改进CT评价［J］．临床放射学杂志，２０００，１９：６５３-６５４．
［７］曾晓华，冯敢生，王颂章，等．建立兔移植性VX２肝癌模型的改进［J］．介入放射学杂志，２００４，１３：５５１-５５３．
［８］柳曦，李欣，赵俊功，等．兔VX２肝癌模型制作及综合影像评价［J］．实用放射学杂志，２００２，１８：１３２-１３４．
［９］吕朋华，王杰，苗毅，等．兔肝VX２转移瘤模型的建立及影像学表现［J］．南京医科大学学报（自然科学版），２００３，２３：５４５-５４７．
［１０］刘瑞宝，徐克，李枫，等．耐药VX２肝癌模型的建立［J］．介入放射学杂志，２００４，１３：３５４-３５７．
［１１］Miao Y，Ni Y，Mulier S，et al．Treatment of VX２liver tumor in rabbits with“wet”electrode mediated radio-frequency ablation［J］．Eur Radiol，２０００，１０：１８８-１９４．
［１２］Pauser S，Wagner S，Lippmann M，et al．Evaluation of efficient chemoembolization mixtures by MR imaging therapy monitoring：an experimental study on the VX２tumor in the rabbit liver［J］，Cancer Res，１９９６，５６：１８６３-１８６７．
［１３］蔡萍，张琳，丁仕义，等．兔VX２肝移植癌模型的建立及CT评价［J］．第三军医大学学报，２００４，２６：１９２４-１９２７．
［１４］张洪新，王执民，曹伟，等．兔VX２移植性肝癌模型的建立及其影像学表现［J］．介入放射学杂志，２００２，１１：１９３-１９６．
［１５］Kuszyk BS，Bluemke DA，Choti MA，et al．Contrast-enhanced CT of small hypovascular hepatic tumor：effect of lesion enhancement on conspicuity in rabbits［J］．AJR，２０００，１７４：４７１-４７５．
［１６］贾洪顺，全显跃，曾盛，等．兔VX２肝癌CT，MRI动态评价［J］．第一军医大学学报，２００２，２２：１４１-１４４．
［１７］Geschwind JH，Artemov D，Abraham S，et al．Chemoemboli-zation of liver tumor in a rabbit modal：assessment of tumor cell death with diffusion-weighted MR imaging and histologic analysis［J］．J Vasc Interv Radiol，２０００，１１：１２４５-１２５５．
［１８］袁友红，肖恩华．肝脏磁共振扩散成像及进展［J］．实用放射学杂志，２００３，１９：９４５-９４８．
［１９］吴红英，冯敢生，梁惠民，等．DSA技术在兔肝脏介入实验中的应用研究［J］．临床放射学杂志，２００５，２４：８４-８５．

相似文献/References:

[1]许永华 朱彤 赵妙嘉 席俊文 张建萍 梁风英.集束电极射频治疗肝恶性肿瘤疗效和并发症的CT评价[J].介入放射学杂志,2002,(01):37.
[2]欧阳墉.经导管肝动脉化疗栓塞术的严重并发症[J].介入放射学杂志,2008,(11):822.
　OUYANG Yong.Severe complications associated with transcatheter hepatic arterial chemoembolization[J].journal interventional radiology,2008,(01):822.
[3]肖运平,肖恩华,罗建光,等.化疗栓塞对肝细胞癌MMP－２、TIMP－２表达的影响[J].介入放射学杂志,2008,(11):784.
　XIAO Yunping,XIAO Enhua,LUO Jianguang,et al.The effect of chemoembolization on MMP－２and TIMP－２expressions of hepatocellular carcinoma[J].journal interventional radiology,2008,(01):784.
[4]关键,胡道予,卢凌,等.经动脉灌注５－FU缓释微球治疗兔VX２肝肿瘤[J].介入放射学杂志,2008,(11):799.
　GUAN Jian,HU Daoyu,LU Ling,et al.Experimental study of transarterial infusion with５－FU sustained－release microspheres for rabbit hepatic VX２tumors[J].journal interventional radiology,2008,(01):799.
[5]邵海波,张曦彤,李红,等.栓塞微球在肝癌介入治疗中的应用[J].介入放射学杂志,2008,(11):776.
　SHAO Haibo,ZHANG Xitong,LI Hong,et al.Application of trisacryl gelatin microspheres in the interventional treatment of hepatic carcinoma[J].journal interventional radiology,2008,(01):776.
[6]罗中华,孙立军,贺洪德,等.术前门静脉栓塞技术与临床[J].介入放射学杂志,2010,(03):248.
　LUO Zhonghua,SUN Lijun,HE Hongde,et al.Preoperative portal vein embolization：the technical and clinical considerations[J].journal interventional radiology,2010,(01):248.
[7]沈海洋,杨光,刘瑞宝,等.原发性肝癌患者肝动脉化疗栓塞术后肝区疼痛的临床意义[J].介入放射学杂志,2010,(04):297.
　SHEN Haiyang,YANG Guang,LIU Ruibao,et al.The clinical significance of liver pain in patients with primary hepatocellular carcinoma after transcatheter arterial chemoembolization[J].journal interventional radiology,2010,(01):297.
[8]潘杰,陈绍辉,卢欣,等.全麻下CT引导下经皮穿刺射频消融治疗肝内特殊部位的恶性肿瘤[J].介入放射学杂志,2010,(06):478.
　PAN Jie,CHEN Shaohui,LU Xin,et al.CT－guided percutaneous radiofrequency ablation of hepatic malignancies located in unusual regions under general anesthesia[J].journal interventional radiology,2010,(01):478.
[9]李国文,梁赵玉,于小平,等.肝转移瘤血供对肝动脉化疗栓塞的近期疗效影响[J].介入放射学杂志,2010,(06):493.
　LI Guowen,LIANG Zhaoyu,YU Xiaoping,et al.The influence of blood supply pattern of hepatic metastases on the short－term effect of TACE[J].journal interventional radiology,2010,(01):493.
[10]申淑群,杨业发,葛乃建,等.肝癌破裂出血的介入与外科手术疗效评价[J].介入放射学杂志,2010,(07):544.
　SHEN Shuqun,YANG Yefa,GE Naijian,et al.Evaluation of interventional and surgical treatments for the hemorrhage of ruptured hepatocellular carcinoma[J].journal interventional radiology,2010,(01):544.
[11]史东宏,曹建民,许健,等.CT引导下兔VX２肝癌模型的制作及综合评估[J].介入放射学杂志,2008,(10):725.
　SHI Donghong,CAO Jianmin,XU Jian,et al.Comprehensive evaluation of establishing rabbit VX２hepatic carcinoma model under CT guidance[J].journal interventional radiology,2008,(01):725.

备注/Memo

备注/Memo:
收稿日期：２００５-１２-２０
基金项目：国家自然科学基金（３０４７０５０７）、江苏省社会发展基金（７７９０００３０３４）
更新日期/Last Update: 2007-01-15