[1]王以浪,王亚非,张 亮,等. 肝细胞癌患者TACE术前后血清miR- 21表达变化及临床意义[J].介入放射学杂志,2014,(05):406-410.
 WANG Yi- lang,WANG Ya- fei,ZHANG Liang,et al. The changes of serum miR- 21 expression level in patients with HCC before and after TACE and its clinical significance[J].journal interventional radiology,2014,(05):406-410.
点击复制

 肝细胞癌患者TACE术前后血清miR- 21表达变化及临床意义()

PDF下载中关闭

分享到:

《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2014年05期
页码:
406-410
栏目:
肿瘤介入
出版日期:
2014-05-28

文章信息/Info

Title:
 The changes of serum miR- 21 expression level in patients with HCC before and after TACE and its clinical significance
作者:
 王以浪 王亚非 张 亮 缪亚军 陈 卓 周 陈 印 滇 丁文彬
Author(s):
 WANG Yi- lang WANG Ya- fei ZHANG Liang MIAO Ya- jun CHEN Zhuo ZHOU Chen YIN Dian DING Wen- bin.
 The First People’s Hospital of Nantong, Natong, Jiangsu Province 226001, China
关键词:
 【关键词】 肝细胞癌 经导管动脉化疗栓塞术 miRNA- 21 血清
文献标志码:
A
摘要:
 【摘要】 目的 探讨HCC患者经皮肝动脉化疗栓塞(TACE)术前、后血清miR- 21表达变化及临床意义。方法 以反转录定量PCR(RT- PCR)法检测HCC患者TACE术前、后及正常者血清miR- 21水平,以酶联免疫吸附法(ELISA)检测血清AFP水平。结果 HCC患者血清miR- 21水平为正常人的(12.9 ± 3.5)倍(t = 19.430 7,P < 0.01),TACE术后1个月为正常参考值的(7.2 ± 1.7)倍,较术前显著降低(t = 9.493 7,P < 0.01)。血清 miR- 21水平与肿瘤大小、癌栓及HBV感染相关。TACE术后1个月血清miR- 21水平在部分缓解、稳定和进展组中分别为正常人的(4.0 ± 0.3)、(6.0 ± 1.5)和(8.6 ± 1.5)倍,各组间差异有统计学意义(F = 38.168,P = 0.000)。miR- 21诊断HCC的ROC- AUC值为0.910 ± 0.041,显著高于AFP的0.860 ± 0.037(t = 6.304 2,P < 0.01)。miR- 21检测HCC的特异度(88.1%)显著高于AFP(69.0%,χ2 = 4.525 3,P = 0.033)。结论 TACE术后miR- 21水平明显降低,能较好预测TACE术疗效,是HCC的潜在分子标记物。

参考文献/References:

 [1] El- Serag HB. Epidemiology of viral hepatitis and hepatocellular carcinoma[J]. Gastroenterology, 2012, 142: 1264 - 1273.e1.
[2] 程永德, 程英升, 颜志平. 常见恶性肿瘤介入治疗指南[M]. 北京: 科学出版社, 2013.
[3] 高 嵩, 朱 旭, 杨仁杰, 等. TACE联合奥沙利铂、 氟尿嘧啶、 亚叶酸钙肝动脉化疗治疗中晚期原发性肝癌[J]. 介入放射学杂志, 2012, 21: 377 - 383.
[4] 梁茂全, 苏洪英. 肝癌化疗栓塞前后甲胎蛋白变化模式的临床意义[J]. 介入放射学杂志, 2012, 21: 333 - 338.
[5] Wang X, Zhang A, Sun H. Power of metabolomics in diagnosis and biomarker discovery of hepatocellular carcinoma[J]. Hepatology, 2013, 57: 2072 - 2077.
[6] Marquardt JU, Galle PR, Teufel A. Molecular diagnosis and therapy of hepatocellular carcinoma (HCC): an emerging field for advanced technologies[J]. J Hepatol, 2012, 56: 267 - 275.
[7] Braconi C, Henry JC, Kogure T, et al. The role of MicroRNAs in human liver cancers[J]. Semin Oncol, 2011, 38: 752 - 763.
[8] Wong CM, Kai AK, Tsang FH, et al. Regulation of hepatocarcinogenesis by microRNAs[J]. Front Biosci (Elite Ed), 2013, 5: 49 - 60.
[9] Schetter AJ, Leung SY, Sohn JJ, et al. MicroRNA expression profiles associated with prognosis and therapeutic outcome in colon adenocarcinoma[J]. JAMA, 2008, 299: 425 - 436.
[10] Karakatsanis A, Papaconstantinou I, Gazouli M, et al. Expression of microRNAs, miR - 21, miR - 31, miR - 122, miR - 145, miR - 146a, miR - 200c, miR - 221, miR - 222, and miR - 223 in patients with hepatocellular carcinoma or intrahepatic cholangiocarcinoma and its prognostic significance[J]. Mol Carcinog, 2013, 52: 297 - 303.
[11] Gabriely G, Wurdinger T, Kesari S, et al. MicroRNA 21 promotes glioma invasion by targeting matrix metalloproteinase regulators[J]. Mol Cell Biol, 2008, 28: 5369 - 5380.
[12] 中国抗癌协会肝癌专业委员会. 中国抗癌协会临床肿瘤学协作专业委员会和中华医学会肝病学分会. 原发性肝癌规范化诊治的专家共识[J]. 实用肝脏病杂志, 2009, 12: 321 - 328.
[13] Jansson MD, Lund AH. MicroRNA and Cancer[J]. Mol Oncol, 2012, 6: 590 - 610.
[14] Zhu Q, Wang Z, Hu Y, et al. miR - 21 promotes migration and invasion by the miR - 21 - PDCD4 - AP - 1 feedback loop in human hepatocellular carcinoma[J]. Oncol Rep, 2012, 27: 1660 - 1668.
[15] Vinciguerra M, Sgroi A, Veyrat - Durebex C, et al. Unsaturated fatty acids inhibit the expression of tumor suppressor phosphatase and tensin homolog (PTEN) via microRNA - 21 up - regulation in hepatocytes[J]. Hepatology, 2009, 49: 1176 - 1184.
[16] Jiang J, Gusev Y, Aderca I, et al. Association of MicroRNA expression in hepatocellular carcinomas with hepatitis infection, cirrhosis, and patient survival[J]. Clin Cancer Res, 2008, 14: 419 - 427.
[17] Knoll S, Fürst K, Thomas S, et al. Dissection of cell context - dependent interactions between HBx and p53 family members in regulation of apoptosis: a role for HBV - induced HCC[J]. Cell Cycle, 2011, 10: 3554 - 3565.
[18] Kew MC. Hepatitis B virus x protein in the pathogenesis of hepatitis B virus - induced hepatocellular carcinoma[J]. J Gastroenterol Hepatol, 2011, 26(suppl 1): 144 - 152.
[19] Qiu X, Dong S, Qiao F, et al. HBx - mediated miR - 21 upregulation represses tumor - suppressor function of PDCD4 in hepatocellular carcinoma[J]. Oncogene, 2013, 32: 3296 - 3305.

相似文献/References:

[1].Spontaneous regression of a giant basilar artery aneurysm in a young adult after surgical injury :case report and literature review[J].介入放射学杂志,2007,(10):718.
 ZHANG He,LI Ming-hua,WANG Chun,et al.Spontaneous regression of a giant basilar artery aneurysm in a young adult after surgical injury :case report and literature review[J].journal interventional radiology,2007,(05):718.
[2]李统怀,郑红枫. 子宫动脉栓塞治疗子宫瘢痕妊娠11例[J].介入放射学杂志,2011,(11):898.
 LI Tong-huai,ZHENG Hong-feng..Clinical application of uterine artery embolization in treating uterine scar pregnancy[J].journal interventional radiology,2011,(05):898.
[3]吕维富,张爱武,周春泽,等. 子宫动脉的三维DSA表现及其最佳显示体位探讨[J].介入放射学杂志,2011,(11):853.
 LV Wei-fu,ZHANG Ai-wu,ZHOU Chun-ze,et al.3D-DSA findings of uterine artery and its optimal projection position[J].journal interventional radiology,2011,(05):853.
[4]张 靖,周少毅,陈昆山,等.经导管动脉硬化栓塞联合注射硬化治疗婴儿颌面部巨大高流量血管瘤[J].介入放射学杂志,2011,(11):848.
 ZHANG Jing,ZHOU Shao-yi,CHEN Kun-shan,et al.Transcatheter arterial sclerosing embolization with injection sclerotherapy for the treatment of huge and high-flow maxillofacial hemangiomas in infants[J].journal interventional radiology,2011,(05):848.
[5]谢小西,吕银祥,章宏欣,等. 肝动脉化疗栓塞、射频消融联合125Ⅰ粒子植入 治疗原发性肝癌的临床应用[J].介入放射学杂志,2011,(11):863.
 XIE Xiao-xi,LV Yin-xiang,ZHANG Hong-xin,et al.The clinical application of TACE together with RFA and 125Ⅰ seed implantation in treating hepatocellular carcinoma[J].journal interventional radiology,2011,(05):863.
[6]王铭义,王 峰. 脑灌注成像在颈动脉狭窄介入治疗中的应用[J].介入放射学杂志,2011,(11):915.
 WANG Ming-yi,WANG Feng..Application of brain perfusion imaging in interventional treatment of carotid artery stenosis[J].journal interventional radiology,2011,(05):915.
[7]杜 猛,张锦生. 介入治疗急性深静脉血栓的临床观察[J].介入放射学杂志,2011,(11):858.
 DU Meng,ZHANG Jing-sheng..Interventional therapy for acute deep venous thrombosis: a clinical observation[J].journal interventional radiology,2011,(05):858.
[8]孟庆娜,李国庆,白晓冬. 急性心肌梗死介入治疗术中恶心呕吐的护理[J].介入放射学杂志,2011,(11):913.
 MENG Qing-na,LI Guo-qing,BAI Xiao-dong..The nursing care of nausea and vomiting occurred in interventional treatment for acute myocardial infarction[J].journal interventional radiology,2011,(05):913.
[9]王海亭,蒋忠仆,周 毅,等. 透视引导下鱼肝油酸钠泡沫硬化剂治疗下肢静脉曲张30例[J].介入放射学杂志,2011,(11):905.
 WANG Hai-ting,JIANG Zhong-pu,ZHOU Yi,et al.Fluoroscopically-guided foam sclerotherapy with sodium morrhuate for the treatment of lower extremity varices[J].journal interventional radiology,2011,(05):905.
[10]杜 鲲,管 生,胡小波,等. 原发性肝癌TACE术后完全缓解病灶磁共振 ADC值可重复性及一致性研究[J].介入放射学杂志,2011,(11):867.
 DU Kun,GUAN Sheng,HU Xiao-bo,et al.Magnetic resonance apparent diffusion coefficient values of the completely remitted lesions of primary hepatocellular carcinoma after TACE: a study of their repeatability and consistency[J].journal interventional radiology,2011,(05):867.
[11]李晓峰,钱国军,张 磊,等. 微波高功率条件下消融原发性肝癌的初步研究[J].介入放射学杂志,2011,(12):974.
 LI Xiao-feng,QIAN Guo-jun,ZHANG Lei,et al.Microwave ablation with high output power for the treatment of hepatocellular carcinoma: a preliminary study[J].journal interventional radiology,2011,(05):974.
[12]姚雪松,李 槐.不可手术切除的肝细胞癌的疗效评价标准——改良RECIST标准更可靠[J].介入放射学杂志,2012,(03):177.
 . Therapeutic evaluation criterion of inoperable hepatocellular carcinomas: modified RECIST as a more reliable standard[J].journal interventional radiology,2012,(05):177.
[13]孙 磊,施海彬,刘 圣,等.肝细胞癌肝动脉门静脉分流形成的相关因素分析[J].介入放射学杂志,2012,(03):206.
 ,,et al.The factors related to the formation of arterioportal shunting in patients with hepatocellular carcinomas [J].journal interventional radiology,2012,(05):206.
[14]梁茂全,苏洪英. 肝癌化疗栓塞前后甲胎蛋白变化模式的临床意义[J].介入放射学杂志,2012,(04):333.
 .The transformation pattern of serum аfetoprotein after transcatheter arterial chemoembolization in patients with hepatocellular carcinoma: its clinical significance [J].journal interventional radiology,2012,(05):333.
[15]沈海洋,刘瑞宝,刘 岩,等. 肝右叶前、后段原发性肝癌TACE后VEGF及CD34的表达水平 ;[J].介入放射学杂志,2012,(06):469.
 SHEN Hai- yang,LIU Rui- bao,LIU Yan,et al. The expression levels of vascular endothelial growth factor and CD34 in residual cancerous tissues of primary hepatocellular carcinoma located at anterior and posterior segments of right lobe liver after TACE[J].journal interventional radiology,2012,(05):469.
[16]彭辽河,胡晓燕,李 杰,等. 18F-FDG PET/CT显像在肝细胞癌TACE术后残留或复发病灶检出中的应用价值[J].介入放射学杂志,2012,(08):636.
 PENG Liao- he,HU Xiao- yan,LI Jie,et al. Clinical application of 18F- FDG PET/CT imaging in detecting residual lesions or recurrence foci of hepatocellular carcinoma after TACE treatment[J].journal interventional radiology,2012,(05):636.
[17]姚雪松,闫 东,曾辉英,等. TACE联合索拉非尼治疗不能手术切除肝细胞肝癌50例[J].介入放射学杂志,2013,(05):381.
 YAO Xue? song,YAN Dong,ZENG Hui? ying,et al. Ttransarterial chemoembolization combined with sorafenib for inoperable hepatocellular carcinoma: a clinical analysis of 50 cases[J].journal interventional radiology,2013,(05):381.
[18]陆小华,朱小庆,茅国新.肝细胞癌相关单核苷酸多态性的研究进展[J].介入放射学杂志,2013,(06):520.
 LU Xiao? hua,ZHU Xiao? qing,MAO Guo? xin.. Hepatocellular carcinoma?蛳 related single nucleotide polymorphisms: recent advances in research[J].journal interventional radiology,2013,(05):520.
[19]赵 松,陈学春,龙清云,等. 经肝动脉化疗栓塞联合射频消融治疗肝细胞癌疗效荟萃分析[J].介入放射学杂志,2013,(11):908.
 ZHAO Song,CHEN Xue? chun,LONG Qing? yun,et al. Transcatheter arterial chemoembolization combined with radiofrequency ablation for the treatment of hepatocellular carcinoma: a systematic review and Meta analysis[J].journal interventional radiology,2013,(05):908.
[20]易长虹,袁琳波,钱 坤,等.肝动脉灌注3-溴丙酮酸阻断大鼠肝癌能量代谢的实验研究[J].介入放射学杂志,2013,(12):1027.
 YI Chang? hong,YUAN Lin? bo,QIAN Kun,et al. Hepatic arterial infusion of 3?蛳 bromopyruvate to block up the energy metabolism of hepatic carcinoma: an experimental study in rats[J].journal interventional radiology,2013,(05):1027.

备注/Memo

备注/Memo:
 (收稿日期:2013-11-13)
更新日期/Last Update: