[1]瞿旭东,王建华,颜志平,等.介入导入三氧化二砷微球对 VX2 兔肝肿瘤模型的治疗作用[J].介入放射学杂志,2007,(03):184.
 QU Xu-dong,WANG Jian-hua,YAN Zhi-ping,et al.The treatment effect of As 2 O 3 -PLGA microspheres on VX2rabbits liver tumor models[J].journal interventional radiology,2007,(03):184.
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介入导入三氧化二砷微球对 VX2 兔肝肿瘤模型的治疗作用 ()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2007年03期
页码:
184
栏目:
实验研究
出版日期:
2007-03-15

文章信息/Info

Title:
The treatment effect of As 2 O 3 -PLGA microspheres on VX2rabbits liver tumor models
作者:
瞿旭东王建华颜志平程洁敏李国平刘嵘钱晟
200032上海复旦大学附属中山医院放射科
Author(s):
QU Xu-dongWANG Jian-huaYAN Zhi-pingCHEN Jie-minLI Guo-pingLIU RongQIAN Cheng
Department Radiology,Zhongshan Hospital,Fudan University,Shanghai200032,China
关键词:
肝细胞肝癌三氧化二砷微球VX2兔
分类号:
R735.7
文献标志码:
A
摘要:
目的观察介入导入三氧化二砷(As 2 O 3 )微球对VX2兔肝肿瘤模型的治疗作用。方法新西兰大白兔32只,体重2.3~2.8kg,制作VX2兔肝肿瘤模型并随机分成4组,每组8只,均经右侧股动脉插管至肝动脉,向肿瘤供血动脉给药:a组动脉灌注组:As 2 O 3 3mg/kg+生理盐水10ml;b组As 2 O 3 微球栓塞组:注入As 2 O 3 PLGA微球3mg/kg;c组空白微球栓塞组:注入PLGA微球3mg/kg;d组为对照组:经肝动脉注入生理盐水10ml。兔肝肿瘤模型制作后14d行肝螺旋CT双期动态扫描,根据螺旋CT扫描获得肝内肿瘤影像,测量肿瘤大小,次日行介入治疗,术后第21天处死实验兔后,取出肝脏,测量瘤体大小;肿瘤组织4%甲醛固定,多点取材,HE染色,显微镜检。结果实验兔介入操作均获成功,且均存活。肿瘤平扫呈低密度,与周围正常肝实质分界欠清。增强后动脉期肿瘤强化明显,坏死组织无强化,呈不均匀高密度,肿瘤与周围肝实质分界清楚。门脉期肿瘤呈不均匀低密度,而周围正常肝实质强化明显,术后CT随访d组和a组肿瘤明显增大,中央呈低密度;c组肿瘤有轻度强化,b组肿瘤体积小,呈低密度,边界清楚,强化不明显。各组肿瘤体积术前无统计学差异,术后标本体积测量显示As 2 O 3 微球栓塞组瘤体最小,a、b、c组与d组间有显著统计学差异(P<0.05);b组与c组、a组间有显著统计学差异(P<0.05)。病理检查显示a组和d组肿瘤体积大,呈鱼肉样,质地脆,坏死区位于肿瘤中心区域,白色豆渣样,肿瘤血管丰富;显微镜下肿瘤细胞丰富,巢团状排列,纤维样组织少与正常肝组织分界不清,呈浸润性生长。b组肿瘤体积小,坏死明显,坏死区边缘纤维组织丰富,在纤维组织内可见残存瘤巢。在纤维组织外围见到肝细胞空泡变性,呈片状分布。结论As 2 O 3 PLGA微球对VX2兔肝肿瘤有良好的化疗栓塞效果,使用安全。

参考文献/References:

[1]王建华,林贵,顾正明,等.肝动脉化疗栓塞术治疗中晚期肝癌[J].中华肿瘤杂志,1992,14:276-277.
[2]Fan J,Ten GJ,He SC,et al.Arterial chemoembolization for hepatocellular carcinoma[J].World J Gastroenterol,1998,4:33-37.
[3]Ebied OM,Federle MP,Carr BI,et al.Evaluation of responses to chemoembolization in patients with unresectable hepatocellular carcinoma[J].Cancer,2003,97:1042-1050.
[4]Ramsey DE,Kernagis LY,Soulen MC,et al.Chemoemboliza-tion of hepatocellular carcinoma[J].J Vasc Interv Radiol,2002,13:S211-S221.
[5]Okada M,Kudo S,Miyazaki O,et al.Antitumoral efficacy and pharmacokinetic properties of pirarubicn upon hepatic intra-arterial injection in the rabbit VX2tumor model[J].Br J Cancer,1995,71:518-524.
[6]Mugitani T,Taniguchi H,Takada A,et al.TNP-470inhibits collateralization to complement the anti-tumor effect of hepatic artery ligation[J].Br J Cancer,1998,77:638-642.
[7]Rous P,Kidd JG,Smith WE.Experiments on the cause of the rabbit carcinomas derived from virus-induced papillomas.II.Loss by the Vx2carcinoma of the power to immunize hosts against the papilloma virus[J].J Exp Med,1952,96:159-174.
[8]Boehm T,Malich A,Goldberg SN,et al.Radio-frequency tumor ablation:internally cooled electrode versus saline-enhanced technique in an aggressive rabbit tumor model[J].Radiology,2002,222:805-813.
[9]Tabaru K,Konno T,Oda T,et al.Treatment of VX2carcinoma implanted in the liver witharterial and intraperitoneal administration of oily anticancer agents[J].Cancer ChemotherPharmacol,2001,47:149-154.
[10]Hamuro M,Nakamura K,Sakai Y,et al.New oily agents for targeting chemoembolization for hepatocellular carcinoma[J].Cardiovasc Intervent Radiol,1999,22:130-134.
[11]邵国良,周康荣,王建华,等.介入治疗实验研究中兔VX2肝癌模型制作的改进和CT评价[J].临床放射学杂志,2000,19:653-655.
[12]田牛,刘育英,李向红,等主编.肝微循环的特点及疾病时的改变:微循环的临床与基础[M].北京:原子能出版社,1996:99.

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备注/Memo

备注/Memo:
收稿日期:2006-12-18
更新日期/Last Update: 2007-03-15