[1]段 林,何艳艳,李天晓,等.涂布镍钛合金表面的人血管内皮细胞生长因子重组质粒转染初步研究[J].介入放射学杂志,2022,31(05):476-479.
DUAN Lin,HE Yanyan,LI Tianxiao,et al.Preliminary study on human VEGF transfected by recombinant plasmid coated with nickel-titanium alloy[J].journal interventional radiology,2022,31(05):476-479.
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涂布镍钛合金表面的人血管内皮细胞生长因子重组质粒转染初步研究()
《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]
- 卷:
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31
- 期数:
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2022年05
- 页码:
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476-479
- 栏目:
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实验研究
- 出版日期:
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2022-07-19
文章信息/Info
- Title:
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Preliminary study on human VEGF transfected by recombinant plasmid coated with nickel-titanium alloy
- 作者:
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段 林; 何艳艳; 李天晓; 陈 松; 贺迎坤; 吴海刚; 卢韬源
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- Author(s):
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DUAN Lin; HE Yanyan; LI Tianxiao; CHEN Song; HE Yingkun; WU Haigang; LU Taoyuan.
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Stroke center,People’s Hospital of Henan University(Henan Provincial People’s Hospital), Zhengzhou, Henan Province 450003, China
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- 关键词:
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【关键词】 血管内皮细胞生长因子; 质粒; 基质胶; 镍钛合金; 转染
- 文献标志码:
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A
- 摘要:
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【摘要】 目的 制备含重组质粒涂层,提高介入医疗器材生物相容性和耐腐蚀性,降低再狭窄率。 方法 将人脐静脉内皮细胞分别接种到涂布基质胶+黏连蛋白和基质胶+黏连蛋白+人血管内皮细胞生长因子(VEGF)重组质粒的镍钛合金丝上,37℃培养48 h后用正置荧光显微镜观测。结果 基质胶+黏连蛋白包被的镍钛合金丝表面细胞无绿色荧光,含人VEGF重组质粒包被的镍钛合金丝表面细胞有绿色荧光,表明人VEGF重组质粒转染成功。结论 人VEGF重组质粒转染成功,此种涂层具有可行性,可用于介入医疗器材表面改性。
参考文献/References:
[1] 雷 毅, 管文婷, 冷 硕, 等. 急性后循环缺血机械取栓研究现状与进展[J]. 介入放射学杂志, 2020, 29: 210- 214.
[2] 张一林, 贺迎坤, 李天晓, 等. 涂布介入器械的人血管内皮生长因子真核表达载体的构建[J]. 中华介入放射学电子杂志, 2019, 7: 40- 43.
[3] Juni RP, Duckers HJ, Vanhoutte PM, et al. Oxidative stress and pathological changes after coronary artery interventions[J]. J Am Coll Cardiol, 2013, 61: 1471- 1481.
[4] 王聪霞, 贾 珊. 冠状动脉支架内再狭窄发生机制的研究进展[J]. 西安交通大学学报(医学版), 2018, 39: 303- 309.
[5] Ye W, Chen Y, Tang W, et al. Reduction- responsive nucleic acid delivery systems to prevent in- stent restenosis in rabbits[J]. ACS Appl Mater Interfaces, 2019, 11: 28307- 28316.
[6] Wu X, Zhao Y, Tang C, et al. Re- endothelialization study on endovascular stents seeded by endothelial cells through up- or downregulation of VEGF[J]. ACS Appl Mater Interfaces, 2016, 8: 7578- 7589.
[7] Vance A, Welch BG. The utility of bioactive coils in the embolization of aneurysms[J]. Neurol Res, 2014, 36: 356- 362.
[8] 张鸿祺, 毛之奇. 神经介入诊断与治疗的发展[J]. 中华神经医学杂志, 2011, 10: 433- 435.
[9] Zhuang Y, Zhang C, Cheng M, et al. Challenges and strategies for in situ endothelialization and long- term lumen patency of vascular grafts[J]. Bioact Mater, 2021, 6: 1791- 1809.
[10] Kandala BSPK, Zhang G, LCorriveau C, et al. Preliminary study on modelling, fabrication by photo- chemical etching and in vivo testing of biodegradable magnesium AZ31 stents [J]. Bioact Mater, 2021, 6: 1663- 1675.
[11] 孔令华, 贺迎坤, 李天晓, 等. 镁基合金可生物降解支架血管内应用研究进展[J]. 介入放射学杂志, 2020, 29: 626- 630.
[12] Ahn SY, Liu J, Vellampatti S, et al. DNA transformations for diagnosis and therapy[J]. Adv Funct Mater, 2020, 2020:2008279.
[13] Zadegan RM, Norton ML. Structural DNA nanotechnology: from design to applications[J]. Int J Mol Sci, 2012, 13: 7149- 7162.
[14] Chandrasekaran AR. Nuclease resistance of DNA nanostructures[J]. Nat Rev Chem, 2021, 5:225-239.
[15] Wang K, Wang H, Li C, et al. Genomic profiling of native R loops with a DNA- RNA hybrid recognition sensor[J]. Sci Adv, 2021, 7: eabe3516.
[16] Wang J, Xu D, Polsky R. Magnetically- induced solid- state electrochemical detection of DNA hybridization[J]. J Am Chem Soc, 2002, 124: 4208- 4209.
[17] Zheng H, Botos I, Clausse V, et al. Conformational constraints of cyclopentane peptide nucleic acids facilitate tunable binding to DNA[J]. Nucleic Acids Res, 2021, 49: 713- 725.
[18] Li X, Liu DR. DNA- templated organic synthesis: nature’s strategy for controlling chemical reactivity applied to synthetic molecules[J]. Angew Chem Int Ed Engl, 2004, 43: 4848- 4870.
[19] Li ZH, Wang J, Li YX, et al. Self- assembled DNA nanoma-terials with highly programmed structures and functions[J]. Mater Chem Front, 2018, 2: 423- 436.
[20] Zha Y, Li Y, Lin T, et al. Progenitor cell- derived exosomes endowed with VEGF plasmids enhance osteogenic induction and vascular remodeling in large segmental bone defects[J]. Theranos- tics, 2021, 11: 397- 409.
[21] Ding L, Hang C, Cheng S,et al. A Soft, conductive external stent inhibits intimal hyperplasia in vein grafts by electropo- ration and mechanical restriction[J]. ACS Nano, 2020, 14:16770- 16780.
备注/Memo
- 备注/Memo:
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(收稿日期:2021- 04- 25)
(本文编辑:边 佶)
更新日期/Last Update:
2022-07-19