[1]王辅明,李娜,罗荣,等.国产冷冻设备（靶向刀）消融活体猪肝的实验研究 [J].介入放射学杂志,2020,29(03):278-282.
　WANG Fuming,LI Na,LUO Rong,et al.Experimental study on cryoablation of pig liver in vivo with domestic cryoablation device[J].journal interventional radiology,2020,29(03):278-282.
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国产冷冻设备（靶向刀）消融活体猪肝的实验研究

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
29
期数:
2020年03
页码:
278-282
栏目:
实验研究
出版日期:
2020-04-20

文章信息/Info

Title:
Experimental study on cryoablation of pig liver in vivo with domestic cryoablation device
作者:
王辅明; 李娜; 罗荣; 韦广旭; 杨继金
Author(s):
WANG Fuming;  LI Na;  LUO Rong;  WEI Guangxu;  YANG Jijin.?
Department of Interventional Radiology, Affiliated Changhai Hospital, Naval Military Medical University, Shanghai 200433, China
关键词:
【关键词】　冷冻消融; 国产; 猪肝; 循环; 消融边缘
文献标志码:
A
摘要:
【摘要】　目的?比较国产冷冻设备（靶向刀）1次冷-热循环和2次冷-热循环的消融范围及实际坏死范围的差异。方法?4只小型巴马猪（25～30 kg），随机分为A、B两组，麻醉后开腹暴露肝脏，放置冷冻针于合适肝叶部位进行冷冻消融，A组选择冷-热（10～3 min）1次循环模式，B组选择冷-热（10～
3 min）2次循环模式。冷循环结束后扫描CT，计算机多平面重建（MPR）描绘出椭球形冰球的最大宽径。术毕处死小型猪，取出靶区肝脏标本，固定48 h后，沿着冷冻针穿刺道垂直方向沿消融区域冰球的最大宽径处切开标本，石蜡包埋切片后进行HE及末端脱氧核苷酸转移酶-生物素dUTP 切口末端标记法(TUNEL)染色。观察消融区域病理学改变，使用Toup View显微镜图像测量软件测量TUNEL染色下坏死区域的边界与针道的距离。结果?1次循环冰球的最大宽径为（27.6±0.9） mm，2次循环的冰球的最大宽径为（31.4±0.5） mm（P＜0.01）。1次循环冰球覆盖范围内实际坏死的边界距冰球覆盖范围的边界距离为（5.6±0.5） mm，2次循环冰球覆盖范围内实际坏死的边界距冰球覆盖范围的边界距离为（3.2±0.2） mm （P＜0.01）。 结论?对于国产冷冻设备（靶向刀） 2次循环较1次循环冰球形成范围更大，且实际坏死区域明显增大，而消融边缘更小。当前2次冷-热循环的消融方案治疗肝脏肿瘤，至少要保证冰球要覆盖病灶周围正常肝脏组织3.5 mm以上才足够。

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备注/Memo

备注/Memo:
（收稿日期：2019-06-14）
（本文编辑：俞瑞纲）
更新日期/Last Update: 2020-04-16