[1]曹 军,何 阳,刘洪强,等.肺癌A549裸大鼠移植瘤模型的建立及应用[J].介入放射学杂志,2014,(10):901-905.
 CAO Jun,HE Yang,LIU Hong qiang,et al.Establishment of nude rat tumor xenograft model of human lung cancer cells A549: its clinical application[J].journal interventional radiology,2014,(10):901-905.
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肺癌A549裸大鼠移植瘤模型的建立及应用 ()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2014年10期
页码:
901-905
栏目:
实验研究
出版日期:
2014-10-25

文章信息/Info

Title:
Establishment of nude rat tumor xenograft model of human lung cancer cells A549: its clinical application
作者:
曹 军 何 阳 刘洪强 王赛博 赵保成 郑晓辉 程英升
Author(s):
CAO Jun HE Yang LIU Hong qiang WANG Sai bo ZHAO Bao cheng ZHENG Xiao hui CHENG Ying sheng.
Medical College, Soochow University, Suzhou, Jiangsu Province 215123, China
关键词:
【关键词】 A549 移植瘤模型 裸大鼠
文献标志码:
A
摘要:
【摘要】 目的 探讨裸大鼠肺癌A549高效移植瘤模型的建立方法,为进行肿瘤的体内相关介入研究提供实验基础。方法 肺癌A549细胞接种于裸小鼠皮下,出瘤后制备单细胞悬液和组织块分别接种和塞入裸大鼠耳后皮下,观察初次培养细胞成瘤组、二次单细胞悬液成瘤组和二次组织块成瘤组的出瘤率和移植瘤的生长情况,流式细胞仪检测细胞周期的变化。结果 3组中二次组织块成瘤组的出瘤率显著高于另外两组,细胞增殖快,移植瘤的变异小;细胞周期分析显示二次组织块成瘤组的细胞S和G2/M期比例增高,细胞增殖指数显著高于另外两组。结论 二次组织块成瘤可显著提高A549的成瘤率,是一种高效的裸大鼠移植瘤模型建立方法。

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备注/Memo

备注/Memo:
(收稿日期:2014-07-09)
(本文编辑:李 欣)
更新日期/Last Update: 2014-10-23