[1]李爱华,高 斌,贺克武,等.椭球形金纳米棒作为癌细胞靶向探针的研究[J].介入放射学杂志,2013,(04):312-316.
 LI Ai? hua,GAO Bin,HE Ke? wu,et al.Ellipsoidal silica- coated gold nanorods used as cancer cell targeting probes: preliminary study[J].journal interventional radiology,2013,(04):312-316.
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椭球形金纳米棒作为癌细胞靶向探针的研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2013年04期
页码:
312-316
栏目:
实验研究
出版日期:
2013-04-17

文章信息/Info

Title:
Ellipsoidal silica- coated gold nanorods used as cancer cell targeting probes: preliminary study
作者:
李爱华 高 斌 贺克武 程继新 肖卫华
Author(s):
LI Ai?蛳 hua GAO Bin HE Ke?蛳 wu CHENG Ji?蛳 xin XIAO Wei?蛳 hua.
School of Medical Imaging, Xuzhou Medical College, Xuzhou, Jiangsu Province 221000, China
关键词:
【关键词】 金纳米棒 二氧化硅 叶酸 荧光染料 细胞摄入
文献标志码:
A
摘要:
【摘要】 目的 制备椭球形核-壳结构二氧化硅包覆的金纳米复合材料。方法 选用二氧化硅作为壳材,制备椭球形二氧化硅包覆的金纳米棒,并用硅烷耦联剂和二氧化硅耦联,将叶酸联接到二氧化硅包覆的金纳米棒(GNRs@SiO2?蛳 FA),用罗丹明异硫氰酸酯与GNRs@SiO2?蛳 FA表面的氨基基团反应,得到罗丹明标记的GNRs@SiO2?蛳 FA。用MTT比色法研究其生物相容性。结果 表面修饰后的金纳米棒有良好的生物相容性。透射电镜观察到金纳米颗粒以细胞吞噬方式进入细胞,可靶向结合于叶酸高表达的癌细胞;激光共聚焦显微镜见GNRs@SiO2?蛳 FA能携带荧光探针进入细胞内,可作为荧光物质或药物的载体。结论 GNRs@SiO2?蛳 FA可靶向作用于癌细胞,并可作为荧光试剂或其他生物标记物的载体,应用于疾病的诊断及肿瘤125I粒子治疗等方面。

参考文献/References:

[1] Zhang X, Xing JZ, Chen J, et al. Enhanced radiation sensitivity in prostate Cancer by gold?蛳 nanoparticles[J]. Clin Invest Med, 2008, 31: E160 ?蛳 E167.[2] Liu C,Wang CH, Chien CC, et al. Enhanced x?蛳 ray irradiation?蛳 induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification[J].Nanotechnology, 2008: 19: 295104.[3] Yang DP, Cui DX. Advances and prospects of Gold nanorods[J]. Chem Asian J, 2008, 3: 2010 ?蛳 2022.[4] Pan B. End?蛳 to?蛳 end self?蛳 assembly and colorimetric characterization of gold nanorods and nanospheres via oligonucleotide hybridiza?蛳 tion[J]. Nanotechnology, 2005, 16: 1776 ?蛳 1780.[5] Eustis S, E1?蛳 Sayed MA. Why gold nano?蛳 particles are more preciousthan pretty gold: noble metalsurface plasmon resonance and its enhancement of the radiative and nonradiative properties of nanocrystals of different shapes[J]. Chem Soc Rev, 2006, 35: 209 ?蛳 217.[6] Liz?蛳 Marzán LM. Tailoring surface plasmons through the morphology and assembly of metal nanoparticles[J]. Langmuir, 2006, 22: 32 ?蛳 41.[7] Tong L, Wei Q, Wei A, et al. Gold nanorods as contrast agents for biological imaging: optical properties, surface conjugation and photothermal effects[J]. Photochem Photobiol, 2009, 85: 21 ?蛳 32.[8] 马占芳, 田 乐, 邸 静, 等. 基于金纳米棒的生物检测、 细胞成像和癌症的光热治疗[J]. 化学进展, 2009, 21: 134 ?蛳 142.[9] Huettmann G. Model system for investigating laser?蛳 induced subcellular microeffects[J]. 2001.[10] Connor EE, Mwamuka J, Gole A, et al. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity[J]. Small, 2005, 1: 325 ?蛳 327.[11] Mirska D, Schirmer K, Fnari SS, et al. Colloids surf[J]. B, 2005, 40: 51 ?蛳 59.[12] Chen ZL, Sun Y, Huang P, et al. Studies on preparation of photosensitizer loaded magnetic silica nanoparticles and their Anti?蛳 Tumor effects for targeting photodynamic therapy[J]. Nanoscale Res Lett, 2009, 4: 400 ?蛳 408.[13] Xie C. Preparation of a novel amino functionalized fluorescein?蛳 doped silica nanoparticle for pH probe[J]. Nano Biomed Eng, 2009, 1: 37.[14] 田 慧, 张 奇, 项光亚, 等. 叶酸受体介导的肿瘤靶向治疗研究进展[J]. 中国临床药理学杂志, 2006, 22: 227 ?蛳 229.[15] Wang CG, Chen Y, Wang TT, et al. Gold nanorods embedded silica particles as Raman label for immunoassay[J]. Adv Funct Mater, 2008, 18: 355 ?蛳 361.[16] Wang CG, Ma ZF, Wang TT, et al. Synthesis, assembly, and biofunctionalization of silica?蛳 coated Gold nanorods for colorimetric biosensing[J]. Adv Funct Mater, 2006, 16: 1673 ?蛳 1678.

备注/Memo

备注/Memo:
(收稿日期:2012-11-18)
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