[1]徐 瀚,周 石,王黎洲,等.索拉非尼对不同人肝癌细胞株中B7- H3和B7- H4蛋白表达的影响 [J].介入放射学杂志,2018,27(01):58-62.
XU Han,ZHOU Shi,WANG Lizhou,et al.The effect of sorafenib on the expressions of B7- H3 and B7- H4 proteins in different human hepatocellular carcinoma cell lines[J].journal interventional radiology,2018,27(01):58-62.
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索拉非尼对不同人肝癌细胞株中B7- H3和B7- H4蛋白表达的影响
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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]
- 卷:
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27
- 期数:
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2018年01期
- 页码:
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58-62
- 栏目:
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实验研究
- 出版日期:
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2018-01-25
文章信息/Info
- Title:
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The effect of sorafenib on the expressions of B7- H3 and B7- H4 proteins in different human hepatocellular carcinoma cell lines
- 作者:
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徐 瀚; 周 石; 王黎洲; 安天志; 蒋天鹏; 宋 杰; 李 兴
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- Author(s):
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XU Han; ZHOU Shi; WANG Lizhou; AN Tianzhi; JIANG Tianpeng; SONG Jie; LI Xing
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Department of Interventional Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province 550004, China
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- 关键词:
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【关键词】 肝癌细胞; B7- H3蛋白; B7- H4蛋白; 免疫印迹法; MTT法
- 文献标志码:
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A
- 摘要:
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【摘要】 目的 研究索拉非尼对不同人肝癌细胞株HepG2、Hep3B、BEL- 7402、BEL- 7404、BEL- 7405、QGY- 7701、 QGY- 7703、SMMC- 7721、MHCC97H、MHCC97L、HCCLM3、HCCLM6中B7- H3和B7- H4蛋白表达的影响。方法 免疫印迹法和溴化噻唑蓝四氮唑(MTT)比色法检测索拉非尼在不同人肝癌细胞株中对B7- H3和B7- H4蛋白表达的影响及索拉非尼对不同人肝癌细胞株的抑制作用。结果 不同人肝癌细胞株中B7- H3和B7- H4蛋白表达较正常人肝细胞(HL- 7702)均显著上调(P<0.01)。索拉非尼对Hep3B、BEL- 7404、MHCC97H、HCCLM3和HCCLM6肝癌细胞株的细胞毒活性IC50值分别为14.56、9.14、9.46、17.21、9.29 μmol/L。分别以5、10、20 μmol/L剂量索拉非尼处理Hep3B、BEL- 7404、MHCC97H、HCCLM3、HCCLM6后,B7- H3和B7- H4蛋白表达均较对照组显著下调(P<0.01)。结论 B7- H3和B7- H4蛋白在不同人肝癌细胞株中通常过表达,可影响肿瘤免疫逃逸,可能是未来肝癌防治的一个新靶点。
参考文献/References:
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备注/Memo
- 备注/Memo:
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(收稿日期:2017-03-30)
(本文编辑:边 佶)
更新日期/Last Update:
2018-01-26