[1]沈 蕾,高 斌,贺克武,等.金纳米棒联合125I粒子诱导肝癌HepG2细胞凋亡的实验研究[J].介入放射学杂志,2015,(03):236-241.
　SHEN Lei,GAO Bin,HE Ke wu,et al.Apoptosis of hepatoma cell line HepG2 induced by the combination use of GNRs@SiO2 FA and 125I seeds： an experimental study[J].journal interventional radiology,2015,(03):236-241.
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金纳米棒联合125I粒子诱导肝癌HepG2细胞凋亡的实验研究 ()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2015年03期
页码:
236-241
栏目:
实验研究
出版日期:
2015-03-25

文章信息/Info

Title:
Apoptosis of hepatoma cell line HepG2 induced by the combination use of GNRs@SiO2 FA and 125I seeds： an experimental study
作者:
沈 蕾;  高 斌;  贺克武;  肖卫华
Author(s):
SHEN Lei;  GAO Bin;  HE Ke wu;  XIAO Wei hua.
Medical Imaging Center， Third Affiliated Hospital， Anhui Medical University， Hefei， Anhui Province 230061， China
关键词:
【关键词】 金纳米棒;  125I粒子;  肝癌;  细胞凋亡;  Bax;  Bcl 2;  Ki67
文献标志码:
A
摘要:
【摘要】 目的 探讨叶酸偶联二氧化硅包覆的金纳米棒（GNRs@SiO2 FA）联合125I粒子诱导肝癌HepG2细胞凋亡及其可能的机制，为临床提高肝癌125I粒子组织间植入疗效提供理论依据。方法 将体外培养的肝癌HepG2细胞随机分成空白对照组，单纯金纳米棒组，单纯125I粒子照射组及金纳米棒联合125I粒子照射组，采用流式细胞仪检测细胞凋亡情况，PT PCR检测bax mRNA、bcl 2 mRNA表达水平，免疫细胞化学检测凋亡相关基因bax、bcl 2表达水平及肿瘤细胞核增殖性抗原Ki67表达情况。结果 流式细胞仪检测4组肝癌HepG2细胞凋亡率，单纯金纳米棒组、单纯125I粒子照射组较空白对照组均有升高（P < 0.05）；联合组较单纯金纳米棒组和单纯125I粒子照射组明显升高，差异有统计学意义（P < 0.05）。联合组bax mRNA表达明显高于单纯金纳米棒组与单纯125I粒子照射组，bcl 2 mRNA表达明显低于单纯金纳米棒组与单纯125I粒子照射组。肝癌HepG2细胞bax表达于胞质，bcl 2表达于胞质和胞膜，Ki67表达于胞核，均表现为棕黄色细颗粒状沉淀。4组肝癌HepG2细胞凋亡相关基因bax、bcl 2及增殖核抗原Ki67蛋白表达量比较，差异均有统计学意义（P < 0.05）；联合组较单纯金纳米棒组和单纯125I粒子照射组Bax蛋白阳性表达率明显升高，Bcl 2、Ki67蛋白阳性表达率明显降低，差异有统计学意义（P < 0.05）。结论 金纳米棒与125I粒子联合应用能更有效增加肝癌细胞凋亡率，其机制可能是通过增加Bax蛋白的表达，抑制Bcl 2蛋白的表达来实现。

参考文献/References:

［1］ Pakin DM, Bray F, Ferlay J, et al. Estimating the world cancer burden: Globocan 2002［J］. CA Cancer J Clin, 2005, 55: 74 75.
［2］ 叶胜龙． 2013年肝癌领域新进展［J］． 中华肝脏病杂志， 2014， 22： 2 4．
［3］ 贺克武， 高 斌， 李嘉嘉． CT导引下125I粒子组织间植入治疗兔VX2肿瘤的实验研究［J］． 介入放射学杂志， 2008， 17： 428 431．
［4］ Lo Muzio L, Staibano S, Pannone G, et al. Expression of theapoptosis inhibitor surviving in aggressive squamous cellcarcinoma［J］. Exp Mol Pathol, 2001, 70: 249 254.
［5］ Ko SH, Ueno T, Yoshimoto Y, et al. Optimizing a novel regional chemotherapeutic agent against melanoma: hyperthermia induced enhancement of temozolomide cytotoxicity［J］. Clin Cancer Res, 2006, 12: 289 297.
［6］ 储玉山， 朱小庆， 张玉宇， 等． 索拉非尼联合奥沙利铂对肝癌HepG2细胞的抑制作用［J］． 介入放射学杂志， 2011， 20： 810 814．
［7］ Skowronek J. Low dose rate or high dose rate bra chytherapy in treatment of prostate cancer between options［J］. J Contemp Brachytherapy, 2013, 5: 33 41.
［8］ Gui C, Cui DX. Functionalized Gold nanorods for tumor imaging and targeted therapy［J］. Cancer Biol Med, 2012, 9: 221 233.
［9］ Roeske JC, Nunez L, Hoggarth M, et al. Characterization of the theorectical radiation dose enhancement from nanoparticles［J］. Technol Cancer Res Treat, 2007, 6: 395 401.
［10］ Cho SH, Jones BL, Krishnan S. The dosimetric feasibility of Gold nanoparticle aided radiation therapy (GNRT) via brachytherapy using low energy gamma /x ray sources［J］. Phys Med Biol, 2009, 54: 4889 4905.
［11］ Park YS, Kasuya A, Dmytruk A, et al. Concentrated colloids of silica encapsulated Gold nanoparticles: colloidal stability, cytotoxicity, and X ray absorption［J］. J Nanosci Nanotechnol, 2007, 7: 2690 2695.
［12］ Conde J, Doria G, Baptista P. Noble metal nanoparticles applications in cancer［J］. J Drug Deliv, 2012: 751075.
［13］ Cory S, Adams JM. Killing cancer cells by flipping the Bcl 2/Bax Switch［J］. Cancer Cell, 2005, 8: 5 6.
［14］ Stefanaki C, Antoniou C, Stefanaki K, et al. Bcl 2 and Bax in congenital naevi［J］. Br J Dermatol, 2006, 154: 1175 1179.
［15］ Yin QH, Yan FX, Zu XY, et al. Anti proliferative and pro apoptotic effect of carvacrol on human hepatocellular carcinoma cell line HepG 2［J］. Cytotechnology, 2012, 64: 43 51.
［16］ Liang CZ, Zhang JK, Shi Z, et al. Matrine induces caspase dependent apoptosis in human osteosarcoma cells in vitro and in vivo through the upregulation of Bax and Fas/FasL and downregulation of Bcl 2［J］. Cancer Chemother Pharmacol, 2012, 69: 317 331.
［17］ Stroescu C, Dragnea A, Ivanov B, et al. Expression of p53, Bcl 2, VEGF, Ki67 and PCNA and prognostic significance in hepatocellular carcinoma［J］. J Gastrointestin Liver Dis, 2008, 17: 411 417.

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备注/Memo

备注/Memo:
（收稿日期：２０１4-０9-05）
（本文编辑：李 欣）
更新日期/Last Update: 2015-03-29