[1]鄂亚军,贺能树,范海伦. 西罗莫司局部缓释对成形术后血管壁ＰＡＩ１及 ｔＰＡ表达的影响[J].介入放射学杂志,2011,(11):885-889.
　E Ya-jun,HE Neng-shu,FAN Hai-lun..The effect of local sustained delivery of sirolimus on the vascular PAI-1 and t-PA expression after angioplasty[J].journal interventional radiology,2011,(11):885-889.
点击复制

  西罗莫司局部缓释对成形术后血管壁ＰＡＩ１及 ｔＰＡ表达的影响 ()

PDF下载中关闭

分享到：

《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2011年11期
页码:
885-889
栏目:
实验研究
出版日期:
2011-11-25

文章信息/Info

Title:
The effect of local sustained delivery of sirolimus on the vascular PAI-1 and t-PA expression after angioplasty
作者:
 鄂亚军;  贺能树;  范海伦
071000 河北保定 河北大学附属医院介入治疗科；天津医科大学总医院放射科
Author(s):
E Ya-jun;  HE Neng-shu;  FAN Hai-lun.
Department of Interventional Radiology, the Affiliated Hospital of Hebei University, Baoding 071000, China
关键词:
【关键词】　　　西罗莫司; 　局部缓释; 　纤溶酶原激活物抑制剂１; 　组织型纤溶酶原激活剂
分类号:
R543
文献标志码:
A
摘要:
【摘要】 目的 探讨西罗莫司局部缓释对成形术后血管壁纤溶酶原激活物抑制剂１（ＰＡＩ１）及组织型纤溶酶原激活剂（ｔＰＡ）表达的影响。方法 建立大鼠颈动脉损伤模型，Ｗｉｓｔａｒ雄性大鼠３０只，分为实验组（２０只）及对照组（１０只）。西罗莫司采取外膜给药，以ｐｌｕｒｏｎｉｃ Ｆ１２７为药物释放载体，给药浓度分为高浓度组６００ μｇ／１００ μｌ及低浓度组３００ μｇ／１００ μｌ，应用免疫组化检测西罗莫司局部缓释对血管壁ＰＡＩ１及ｔＰＡ表达的影响。结果 损伤后１５及３０ ｄ，西罗莫司高、低浓度实验组均促进新生内膜中ＰＡＩ１的表达（Ｐ ＜ ０．０５），对中膜ＰＡＩ１表达的影响除高浓度１５ ｄ组与对照相比无统计学差异（Ｐ ＞ ０．０５）外，其余各组均高于对照组（Ｐ ＜ ０．０５）。损伤后１５及３０ ｄ，西罗莫司实验组新生内膜ｔＰＡ的表达低于对照组（Ｐ ＜ ０．０５），而中膜ｔＰＡ的表达高浓度 １５ ｄ及３０ ｄ组低于对照组（Ｐ ＜ ０．０５），低浓度组与对照组相比无统计学差异（Ｐ ＞ ０．０５）。结论 西罗莫司局部缓释在抑制新生内膜增殖的同时亦造成局部ＰＡＩ１的高表达及ｔＰＡ的较低表达，两者表达的失衡可能成为晚期血栓形成的重要原因。

参考文献/References:

[1] Slavin L, Chhabra A, Tobis JM. Drug-eluting stents: preventing restenosis[J]. Cardiol Rev, 2007, 15: 1 - 12.
[2] Stone GW, Ellis SG, Cox DA. A polymer-based, paclitaxel-eluting stent in patients with coronary artery disease [J]. N Engl J Med, 2004, 350: 221 - 231.
[3] Moses JW, Leon MB, Popma JJ, et al. Sirolimus-eluting stents versus standard stents in patients with stenosis in a native coronary artery[J]. N Engl J Med, 2003, 349: 1315 - 1323.
[4] 韩 健, 郭富强. 经皮腔内血管成形术后再狭窄的炎症预测因子[J]. 介入放射学杂志, 2009, 18: 876 - 879.
[5] 张 琳, 张瑞岩. 药物洗脱支架术后再内皮化延迟与晚期血栓形成[J]. 国际心血管病杂志, 2009, 36: 91 - 93, 97.
[6] Shuchman M. Trading restenosis for thrombosis?New questions about drug-eluting stents[J]. N Engl J Med, 2006, 355: 1949 - 1952.
[7] Maisel WH. Unanswered questions--drug-eluting stents and the risk of late thrombosis[J]. N Engl J Med, 2007, 356: 981 - 984.
[8] Bucalo R, Capranzano P, Conti G, et al. Sirolimus versus paclitaxel-eluting stents in small coronary vessels: long-term outcomes from a single-center registry[J]. J Cardiovasc Med (Hagerstown), 2010, 11: 365 - 368.
[9] Muldowney JA 3rd, Stringham JR, Levy SE, et al. Antipro-liferative agents alter vascular plasminogen activator inhibitor-1 expression: a potential prothrombotic mechanism of drug-eluting stents[J]. Arterioscler Thromb Vasc Biol, 2007, 27: 400 - 406.
[10] Pontrelli P, Rossini M, Infante B, et al. Rapamycin inhibits PAI-1 expression and reduces interstitial fibrosis and glomerulos-clerosis in chronic allograft nephropathy[J]. Transplantation, 2008, 85: 125 - 134.
[11] Clowes AW, Reidy M, Clowes MM. Mechanisms of stenosis after arterial injury[J]. Lab Invest, 1983, 49: 208 - 215.
[12] 王永利, 贺能树, 张家兴, 等. 血管成形术后外膜细胞表型转化和迁移的实验研究[J]. 介入放射学杂志, 2006, 15: 424 - 429.
[13] Lang NN, Newby DE. Emerging thrombotic effects of drug eluting stents[J]. Arterioscler Thromb Vasc Biol, 2007, 27: 261 - 262.
[14] Rosenberg RD, Aird WC. Vascular-bed--specific hemostasis and hypercoagulable states[J]. N Engl J Med, 1999, 340: 1555 - 1564.
[15] Joner M, Finn AV, Farb A, et al. Pathology of drug-eluting stents in humans: delayed healing and late thrombotic risk[J]. J Am Coll Cardiol, 2006, 48: 193 - 202.
[16] Urbich C, Dimmeler S. Endothelial progenitor cells functional characterization[J]. Trends Cardiovasc Med, 2004, 14: 318 - 322.
[17] Bruemmer D, Yin F, Liu J, et al. Rapamycin inhibits E2F-dependent expression of minichromosome maintenance proteins in vascular smooth muscle cells[J]. Biochem Biophys Res Commun, 2003, 303: 251 - 258.
[18] Koziczak M, Müller H, Helin K, et al. E2F1-mediated transcriptional inhibition of the plasminogen activator inhibitor type 1 gene[J]. Eur J Biochem, 2001, 268: 4969 - 4978.
[19] Lim Y, Kim TJ, Jin YR, et al. Epothilone B inhibits neointimal formation after rat carotid injury through the regulation of cell cycle-related proteins[J]. J Pharmacol Exp Ther, 2007, 321: 648 - 655.
[20] Ishizaka N, Taguchi J, Kimura Y, et al. Effects of a single local administration of cilostazol on neointimal formation in balloon-injured rat carotid artery[J]. Atherosclerosis, 1999, 142: 41 - 46.



 

备注/Memo

备注/Memo:
(收稿日期:2011-06-16)
更新日期/Last Update: