[1]张 靖,姜 华,陈昆山,等.经眼动脉灌注治疗兔成视网膜细胞瘤 [J].介入放射学杂志,2015,(02):146-149.
　ZHANG Jing,JIANG Hua,CHEN Kun shan,et al.Selective ophthalmic arterial perfusion of melphalan for retinoblastoma： an experimental study in rabbits[J].journal interventional radiology,2015,(02):146-149.
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经眼动脉灌注治疗兔成视网膜细胞瘤 ()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2015年02期
页码:
146-149
栏目:
实验研究
出版日期:
2015-02-25

文章信息/Info

Title:
Selective ophthalmic arterial perfusion of melphalan for retinoblastoma： an experimental study in rabbits
作者:
张 靖;  姜 华;  陈昆山;  李海波;  蒋贻洲;  林雀卿;  李 丹;  黄晓明;  肖湘生
Author(s):
ZHANG Jing;  JIANG Hua;  CHEN Kun shan;  LI Hai bo;  JIANG Yi zhou;  LIN Que qing;  LI Dan;  HUANG Xiao ming;  XIAO Xiang sheng.
Department of Radiology， Affiliated Changzheng Hospital， Second Military Medical University， Shanghai 200003， China
关键词:
【关键词】 成视网膜细胞瘤;  动物模型;  经眼动脉灌注术;  美法仑
文献标志码:
A
摘要:
【摘要】 目的 评估兔成视网膜细胞瘤（retinoblastoma，RB）模型的建立及经导管眼动脉灌注（selective ophthalmic arterial injection，SOAI）治疗兔RB的可行性。方法 HXO Rb44RB细胞予中心细胞室培养，取对数生长期HXO Rb44细胞，用PBS配成浓度为2.0 × 107个/ml的细胞悬液，抽取0.1 ml细胞悬液注射于6只SPF级新西兰兔右眼视网膜间隙下。接种肿瘤细胞前3 d和注射后1个月臀部注射环孢素A 15 mg?kg-1?d-1，1个月后减量至10 mg?kg-1?d-1并持续到实验结束。将RB兔分为对照组3只和实验组3只，对照组灌注0.9%氯化钠溶液15 ml，实验组将美法仑2.5 mg用0.9%氯化钠溶液稀释至15 ml后灌注。术后分别于7、14 d比较两组肿瘤生长情况。结果 注射环孢素A期间，实验兔不良反应轻微，6只均建模成功。7次插管中6次成功插至眼动脉。SOAI术后1周，实验组肿瘤可见明显缩小，2周后均消失。SOAI术后，实验组仅1只见右眼轻度红肿、流泪，1周后红肿自行消退。结论 SOAI治疗RB兔具有可行性，为进一步经眼动脉灌注治疗RB的基础研究提供了参考。

参考文献/References:

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备注/Memo

备注/Memo:
（收稿日期：２０１4-０8-０3）
（本文编辑：李 欣）
更新日期/Last Update: 2015-02-16