[1]田 伟,黄学卿,王黎洲,等.鞣花酸经活性氧与血管再生途径对四氯化碳诱导肝硬化的保护作用研究[J].介入放射学杂志,2018,27(04):357-362.
 TIAN Wei,HUANG Xueqing,WANG Lizhou,et al.The protective effects of ellagic acid on carbon tetrachloride- induced cirrhosis through reactive oxygen species and angiogenesis ways[J].journal interventional radiology,2018,27(04):357-362.
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鞣花酸经活性氧与血管再生途径对四氯化碳诱导肝硬化的保护作用研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
27
期数:
2018年04期
页码:
357-362
栏目:
实验研究
出版日期:
2018-04-25

文章信息/Info

Title:
The protective effects of ellagic acid on carbon tetrachloride- induced cirrhosis through reactive oxygen species and angiogenesis ways
作者:
田 伟 黄学卿 王黎洲 周 石 安天志 宋 杰 蒋天鹏
Author(s):
TIAN Wei HUANG Xueqing WANG Lizhou ZHOU Shi AN Tianzhi SONG Jie JIANG Tianpeng
Department of Interventional Radiology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province 550004, China
关键词:
【关键词】 鞣花酸 四氯化碳 肝硬化 活性氧 血管再生
文献标志码:
A
摘要:
【摘要】 目的 研究鞣花酸(EA)对四氯化碳(CCl4)诱导的肝硬化小鼠模型的保护作用,并探讨其作用机制。方法 将40只小鼠随机分为对照组(n=10,予0.9%氯化钠溶液)和肝硬化模型组(n=30,由橄榄油稀释10%CCl4诱导建模),模型组又随机分为EA未干预组(n=10)、7.5 mg/kg EA组(n=10)、15 mg/kg EA组(n=10)。检测所有小鼠血清谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和白蛋白(ALB)水平,苏木精-伊红(HE)染色观察肝细胞形态。采用逆转录-聚合酶链反应(RT- PCR)检测肝细胞Ⅰ型胶原蛋白mRNA水平,酶联免疫吸附试验(ELISA)检测谷胱甘肽过氧化物酶(GSH- PX)、谷胱甘肽(GSH)、超氧化物歧化酶(SOD)、丙二醛(MDA)及半胱氨酸天冬氨酸特异性蛋白酶(caspase)- 3活性,蛋白印迹检测诱导型一氧化氮合酶(iNOS)、血管内皮细胞生长因子(VEGF)、VEGF受体(VEGFR)- 2表达水平。结果 根据肝细胞ALT、AST、ALB含量检测及HE染色细胞形态观察结果,推测EA对CCl4诱导的肝硬化小鼠具有保护作用。EA能显著抑制Ⅰ型胶原和iNOS表达,氧化应激及活性氧(ROS)形成显著降低,EA干预组VEGF、VEGFR- 2表达及caspase- 3活性显著提高。结论 EA通过ROS及血管再生途径对CCl4诱导的肝硬化有保护作用。

参考文献/References:

[1] 王 磊, 刘福全, 岳振东, 赵洪伟. 应用介入技术建立猪肝硬化门脉高压模型的实验研究[J]. 介入放射学杂志, 2012, 21: 756- 759.
[2] Caprai S, Vajro P, Ventura A, et al. Autoimmune liver disease associated with celiac disease in childhood: a multicenter study[J]. Clin Gastroenterol Hepatol, 2008, 6: 803- 806.
[3] Assy N, Kayal M, Mejirisky Y, et al. The changes in renal function after a single dose of intravenous furosemide in patients with compensated liver cirrhosis[J]. BMC Gastroenterol, 2006, 6: 39- 43.
[4] Kuiper EM, Hansen BE, Lesterhuis W, et al. The long- term effect of ursodeoxycholic acid on laboratory liver parameters in biochemically non- advanced primary biliary cirrhosis[J]. Clin Res Hepatol Gastroenterol, 2011, 35: 29- 33.
[5] Misik M, Hoelzl C, Wagner KH, et al. Impact of paper filtered coffee on oxidative DNA- damage: results of a clinical trial[J]. Mutat Res, 2010, 692: 42- 48.
[6] Spahr L, Bresson- Hadni S, Amann P, et al. Allopurinol, oxidative stress and intestinal permeability in patients with cirrhosis: an open- label pilot study[J]. Liver Int, 2007, 27: 54- 60.
[7] Simon- Talero M, Garcia- Martinez R, Torrens M, et al. Effects of intravenous albumin in patients with cirrhosis and episodic hepatic encephalopathy: a randomized double- blind study[J]. J Hepatol, 2013, 59: 1184- 1192.
[8] Deng G, Wang J, Zhang Q, et al. Hepatoprotective effects of phloridzin on hepatic fibrosis induced by carbon tetrachloride against oxidative stress- triggered damage and fibrosis in rats[J]. Biol Pharm Bull, 2012, 35: 1118- 1125.
[9] Fang H, Liu A, Dirsch O, et al. Liver transplantation and inflammation: is lipopolysaccharide binding protein the link? [J]. Cytokine, 2013, 64: 71- 78.
[10] Marotta F, Yoshida C, Barreto R, et al. Oxidative- inflammatory damage in cirrhosis: effect of vitamin E and a fermented papaya preparation[J]. J Gastroenterol Hepatol, 2007, 22: 697- 703.
[11] Modi M, Goel T, Das T, et al. Ellagic acid & gallic acid from Lagerstroemia speciosa L. inhibit HIV- 1 infection through inhibition of HIV- 1 protease & reverse transcriptase activity[J]. Indian J Med Res, 2013, 137: 540- 548.
[12] Ural MS, Yonar ME, Mise Yonar S. Protective effect of ellagic acid on oxidative stress and antioxidant status in Cyprinus carpio during malathion exposure[J]. Cell Mol Biol(Noisy- le- grand), 2015, 61: 58- 63.
[13] Mishra S, Vinayak M. Role of ellagic acid in regulation of apoptosis by modulating novel and atypical PKC in lymphoma bearing mice[J]. BMC Complement Altern Med, 2015, 15: 281
[14] Zhang HM, Zhao L, Li H, et al. Research progress on the anticarcinogenic actions and mechanisms of ellagic acid[J]. Cancer Biol Med, 2014, 11: 92- 100.
[15] De Minicis S, Brenner DA. Oxidative stress in alcoholic liver disease: role of NADPH oxidase complex[J]. J Gastroenterol Hepatol, 2008, 23(Suppl 1): S98- S103.
[16] Oettl K, Stadlbauer V, Petter F, et al. Oxidative damage of albumin in advanced liver disease[J]. Biochim Biophys Acta, 2008, 1782: 469- 473.
[17] Vidali M, Occhino G, Ivaldi A, et al. Combination of oxidative stress and steatosis is a risk factor for fibrosis in alcohol- drinking patients with chronic hepatitis C[J]. Am J Gastroenterol, 2008, 103: 147- 153.
[18] Li J, Wang Y. Effect of different methods of hypoxic exercise training on free radical oxidation and antioxidant enzyme activity in the rat brain[J]. Biomed Rep, 2013, 1: 925- 929.
[19] Yan Z, Qu K, Zhang J, et al. CD147 promotes liver fibrosis progression via VEGF- A/VEGFR2 signalling- mediated cross- talk between hepatocytes and sinusoidal endothelial cells[J]. Clin Sci (Lond), 2015, 129: 699- 710.
[20] Tekkesin N, Taga Y, Sav A, et al. Induction of HGF and VEGF in hepatic regeneration after hepatotoxin- induced cirrhosis in mice[J]. Hepatogastroenterology, 2011, 58: 971- 979.
[21] Kishta SA, Abd- Alhade AA, Hamam O, et al. Prognostic value of TNF a mRNA and VEGF mRNA expression in patients with chronic hepatitis C genotype- 4, with and without cirrhosis and hepatocellular carcinoma to predict disease outcome[J]. J Egypt Soc Parasitol, 2010, 40: 515- 530.
[22] Jaroszewicz J, Januszkiewicz M, Flisiak R, et al. Circulating vascular endothelial growth factor and its soluble receptors in patients with liver cirrhosis: possible association with hepatic function impairment[J]. Cytokine, 2008, 44: 14- 17.
[23] Mukozu T, Nagai H, Matsui D, et al. Serum VEGF as a tumor marker in patients with HCV- related liver cirrhosis and hepatocellular carcinoma[J]. Anticancer Res, 2013, 33: 1013- 1021.
[24] Surapaneni KM, Vishnu Priya V, Mallika J. Effect of pioglitazone, quercetin, and hydroxy citric acid on vascular endothelial growth factor messenger RNA(VEGF mRNA) expression in experimentally induced nonalcoholic steatohepatitis(NASH)[J]. Turk J Med Sci, 2015, 45: 542- 546.
[25] Labrecque L, Lamy S, Chapus A, et al. Combined inhibition of PDGF and VEGF receptors by ellagic acid, a dietary- derived phenolic compound[J]. Carcinogenesis, 2005, 26: 821- 826.
[26] Izawa T, Horiuchi T, Atarashi M, et al. Anti- fibrotic role of miR- 214 in thioacetamide- induced liver cirrhosis in rats[J]. Toxicol Pathol, 2015, 43: 844- 851.
[27] Liu C, Wang G, Chen G, et al. Huangqi decoction inhibits apoptosis and fibrosis, but promotes Kupffer cell activation in dimethylnitrosamine- induced rat liver fibrosis[J]. BMC Complement Altern Med, 2012, 12: 51.
[28] 初金哲, 王黎洲, 张 帅, 等. 改良的经大鼠胃十二指肠动脉逆行肝动脉插管实验研究[J]. 介入放射学杂志, 2016, 25: 798- 802.

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备注/Memo

备注/Memo:
(收稿日期:2017-05-22)
(本文编辑:边 佶)
更新日期/Last Update: 2018-04-16