[1]黄浙勇,葛均波,杨姗,等.铁羧葡胺标记猪间充质干细胞的体内外磁共振成像研究[J].介入放射学杂志,2007,(02):115.
HUANG Zhe-yong,GE Jun-bo,YANG Shan,et al.In vitro and in vivo MR imaging of SHU555A-labelled swine bone marrow mesenchymal stem cells[J].journal interventional radiology,2007,(02):115.
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铁羧葡胺标记猪间充质干细胞的体内外磁共振成像研究()
《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]
- 卷:
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- 期数:
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2007年02期
- 页码:
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115
- 栏目:
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实验研究
- 出版日期:
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2007-02-15
文章信息/Info
- Title:
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In vitro and in vivo MR imaging of SHU555A-labelled swine bone marrow mesenchymal stem cells
- 作者:
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黄浙勇; 葛均波; 杨姗; 等
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200032上海复旦大学附属中山医院心内科/上海市心血管病研究所(黄浙勇、葛均波、张少衡、黄榕翀、孙爱军、钱菊英、邹云增);放射科(杨姗、金航、曾蒙苏)
- Author(s):
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HUANG Zhe-yong; GE Jun-bo; YANG Shan; ZHANG Shao-heng; HUANG Rong-chong; ZENG Meng-su; JIN Hang; SUN Ai-jun; QIAN Ju-ying; ZOU Yun-zeng.
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Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,Fudan University,Shanghai200032,China
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- 关键词:
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超顺磁性氧化铁; 间充质干细胞; 磁共振成像; 铁羧葡胺
- 分类号:
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R541
- 文献标志码:
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A
- 摘要:
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目的探讨铁羧葡胺-多聚赖氨酸复合物标记猪骨髓间充质干细胞(MSC)的体外和活体心脏内MR成像的特点。方法分离培养猪MSC,用含铁羧葡胺-多聚赖氨酸复合物标记细胞24h。分别于标记后0、4、8、12、16、20d行普鲁士蓝染色观察细胞内铁,原子吸收分光光度仪测定细胞内含铁量,锥虫蓝排除试验检测细胞活力。对不同时间点、不同细胞数的磁标记干细胞进行1.5T MR仪体外成像,并对植入猪心肌内的标记细胞进行体内MR成像。结果①MSC标记后见胞质中大量普鲁士蓝着色颗粒,标记率达100%,铁离子含量平均为(13.13±2.30)pg/细胞;随细胞的分裂增殖,细胞内铁离子含量逐渐减少,16d时铁离子含量下降到(0.68±0.20)pg/细胞,接近标记前水平眼穴0.21±0.06雪pg/细胞,P>0.05演。干细胞磁标记后各时间点的锥虫蓝拒染率与未标记细胞无统计学差异(P>0.05)。②3种成像序列中GRE T
2
觹WI信号改变最为明显,成像细胞数量越多,信号强度变化越明显。1×10
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个细胞进行MR成像,发现随着标记后体外培养时间延长,T
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觹WI磁标低信号逐渐消失,12d以后信号强度和标记前无差异(P>0.05)。体外MR成像最少能检测到5×10
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~1×10
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个标记的猪MSC。③标记细胞心肌内移植后1周MR成像显示低信号区。结论应用铁羧葡胺-多聚赖氨酸复合物标记猪MSC安全、高效;体外MR信号强度能一定程度上反映磁标记细胞的数量及增殖情况;1.5T临床MR成像仪可对植入心脏的磁标记细胞进行活体显像示踪。
参考文献/References:
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备注/Memo
- 备注/Memo:
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收稿日期:2006-10-09
基金项目:“十五”国家科技攻关计划项目(2004BA714B05-2);上海市科委优秀学科带头人计划(03XD14010)
更新日期/Last Update:
2007-02-15