[1]朱光宇,卢勤,滕皋军,等.经导管动脉注入脂质体介导的p53基因治疗肝癌的实验研究[J].介入放射学杂志,2007,(02):109.
 ZHU Guang-yu,LU Qin,TENG Gao-jun,et al.Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53genes[J].journal interventional radiology,2007,(02):109.
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经导管动脉注入脂质体介导的 p53 基因治疗肝癌的实验研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2007年02期
页码:
109
栏目:
实验研究
出版日期:
2007-02-15

文章信息/Info

Title:
Experimental research on treating hepatic carcinoma by arterial injection of liposome mediated p53genes
作者:
朱光宇 卢勤 滕皋军 魏晓莹 郭金和 余辉 邓钢 何仕诚 方文 李国昭
210009南京东南大学附属中大医院放射科(朱光宇、卢勤、滕皋军、郭金和、余辉、邓钢、何仕诚、方文、李国昭),病理科(魏晓莹)
Author(s):
ZHU Guang-yuLU QinTENG Gao-junWEI Xiao-yingGUO Jin-heYU HuiDENG GangHE Shi-chengFANG WenLI Guo-zhao
Department of Radiology,Pathology,Zhongda Hospital,Southeast University,Nanjing210009,China
关键词:
p53基因脂质体转染效率肝癌放射学介入性
分类号:
R735.7
文献标志码:
A
摘要:
目的以兔VX2肝癌模型为对象,探讨经导管动脉注入脂质体介导的p53基因治疗肝癌的可行性及转染和表达情况。方法将pCMV-myc-p53质粒、阳离子脂质体LipofectAMINE以及pCMV-myc-p53和LipofectAMINE的复合体分别注入兔VX2肝癌模型的肿瘤供血动脉,并提取肿瘤组织蛋白,采用蛋白印迹法及免疫组化检测基因转染及其表达。以不同量的pCMV-myc-p53与LipofectAMINE形成的复合体分别注入兔VX2肝癌模型的肿瘤供血动脉内,同法检测基因的转染及其表达。结果脂质体介导的p53基因经动脉途径成功转染了兔VX2肝癌模型的肿瘤组织并进行表达,其转染效率明显高于单纯基因导入,基因的量与转染效率之间存在量效关系。结论经动脉途径导入脂质体介导的p53基因治疗肝癌是可行、有效的,具有广阔的应用前景。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2006-10-12
更新日期/Last Update: 2007-02-15