[1]杨 钦,程芝梅,王黎洲,等.利多卡因-表柔比星-超液化碘油混合乳剂在体外及兔肝癌模型中缓释效果的研究[J].介入放射学杂志,2023,32(01):43-47.
 YANG Qin,CHENG Zhimei,WANG Lizhou,et al.The slow-release effect of lidocaine-epirubicin-superliquefied lipiodol mixed emulsion in vitro and in rabbit liver cancer models[J].journal interventional radiology,2023,32(01):43-47.
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利多卡因-表柔比星-超液化碘油混合乳剂在体外及兔肝癌模型中缓释效果的研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
32
期数:
2023年01
页码:
43-47
栏目:
实验研究
出版日期:
2023-02-13

文章信息/Info

Title:
The slow-release effect of lidocaine-epirubicin-superliquefied lipiodol mixed emulsion in vitro and in rabbit liver cancer models
作者:
杨 钦 程芝梅 王黎洲 蒋天鹏 周 石
Author(s):
YANG Qin CHENG Zhimei WANG Lizhou JIANG Tianpeng ZHOU Shi.
School of Medical Imaging, Guizhou Medical University, Guiyang, Guizhou Province 550004, China
关键词:
【关键词】肝动脉化疗栓塞术利多卡因兔VX2肝癌模型超高效液相色谱-质谱联用法
文献标志码:
A
摘要:
【摘要】 目的 探讨利多卡因-表柔比星-超液化碘油混合乳剂在体外缓释效果,及兔VX2肝癌模型经肝动脉化疗栓塞中利多卡因与碘油乳化后,肝癌组织中利多卡因的动态分布情况。方法 采用W/O法对利多卡因-表柔比星乳剂进行体外理化实验,并在72 h内测定利多卡因-表柔比星-碘油乳剂的累积缓释量。随机选取48只新西兰白兔,超声引导下经皮穿刺建立兔VX2肝癌模型,分为实验组和对照组,每组24只,实验组以利多卡因-表柔比星-碘油混合乳剂进行栓塞,对照组先予以动脉内灌注利多卡因,再以表柔比星-碘油混合乳剂行栓塞,分别于术后的0.5、1、4、8、24、48、96、144 h时间点处死实验组及对照组各3只兔子,取肝癌组织,经UPLC-MS/MS测定癌组织中利多卡因药物浓度,通过Graphpad软件拟合肝癌组织中的利多卡因药物浓度随时间变化曲线。采用独立样本t检验比较TACE中利多卡因油包水技术和单纯灌注后肝癌组织中利多卡因的动态变化情况。结果 体外实验中,利多卡因-表柔比星-碘油乳剂0~72 h内利多卡因浓度达到T50%约需30 min。24 h内利多卡因释放量超过80%。动物实验中,VX2肝癌模型建模成功率和TACE术成功率均为100%。与对照组相比,实验组兔肝癌组织中利多卡因的药物浓度量显著增加、清除显著降低,肿瘤组织内的利多卡因停留时间延长。结论 利多卡因-表柔比星-碘油乳剂对利多卡因具有缓释作用,利多卡因与碘油乳化后经TACE治疗可增加各时间段组织中利多卡因的浓度,验证了利多卡因混合碘油后在肝脏内血管具有缓慢释放的效果。

参考文献/References:

[1] Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019[J]. CA Cancer J Clin, 2019, 69: 7-34.
[2] Forner A, Reig M, Bruix J. Hepatocellular carcinoma[J]. Lancet,2018, 391:1301-1314.
[3] Wang A, Wu L, Lin J,et al. Whole-exome sequencing reveals the origin and evolution of hepato-cholangiocarcinoma[J]. Nat Commun, 2018, 9:894.
[4] Chang Lee R, Tebbutt N. Systemic treatment of advanced hepatocellular cancer: new hope on the horizon[J]. Expert Rev Anticancer Ther, 2019, 19:343-353.
[5] Torre LA, Bray F, Siegel RL, et al. Global cancer statistics,2012[J]. CA Cancer J Clin, 2015, 65: 87-108.
[6] Reynolds AR, Furlan A, Fetzer DT, et al. Infiltrative hepatocellular carcinoma: what radiologists need to know[J]. Radiographics, 2015: 371-386.
[7] Khalaf MH, Sundaram V, Abdelrazek MM, et al. A predictive model for postembolization syndrome after transarterial hepatic chemoembolization of hepatocellular carcinoma[J]. Radiology, 2019, 290: 254-261.
[8] Lv N, Kong Y, Mu L, et al. Effect of perioperative parecoxib sodium on postoperative pain control for transcatheter arterialhemoembolization for inoperable hepatocellular carcinoma: aprospective randomized trial[J]. Eur Radiol, 2016, 26: 3492-3499.
[9] Kanematsu M, Semelka RC, Leonardou P, et al. Hepatocellular carcinoma of diffuse type: MR imaging findings and clinical manifestations[J]. J Magn Reson Imaging, 2003, 18: 189-195.
[10] Yau T, Tang V, Yao TJ, et al. Development of Hong Kong liver cancer staging system with treatment stratification for patients with hepatocellular carcinoma[J]. Gastroenterology, 2014, 146: 1691-1700.
[11] Dai QS, Gu HL, Ye S, et al. Transarterial chemoembolization vs. conservative treatment for unresectable infiltrating hepatocellular carcinoma: a retrospective comparative study[J]. Mol Clin Oncol,2014, 2: 1047-1054.
[12] Jang ES, Yoon JH, Chung JW, et al. Survival of infiltrative hepatocellular carcinoma patients with preserved hepatic function after treatment with transarterial chemoembolization[J]. J Cancer Res Clin Oncol, 2013, 139: 635-643.
[13] Lee SH, Hahn ST, Park SH. Intraarterial lidocaine administration for relief of pain resulting from transarterial chemoembolization of hepatocellular carcinoma: its effectiveness and optimal timing of administration[J]. Cardiovasc Intervent Radiol, 2002, 24: 368-371.
[14] Nault JC, Nkontchou G, Nahon P, et al. Percutaneous treatment of localized infiltrative hepatocellular carcinoma developing on cirrhosis[J]. Ann Surg Oncol, 2016, 23: 1906-1915.
[15] Duan F, Bai YH, Cui L, et al. Transarterial embolization with N-butyl 2-cyanoacrylate for the treatment of arterioportal shunts in patients with hepatocellular carcinoma[J]. J Cancer Res Ther, 2017, 13: 631-635.
[16]Sieghart W, Hucke F, Peck-Radosavljevic M. Transarterial chemoembolization: modalities, indication,and patient selection[J]. J Hepatol, 2015, 62: 1187-1195.
[17] Masada T, Tanaka T, Nishiofuku H, et al. Techniques to form a suitable lipiodol-epirubicin emulsion by using 3-way stopcock methods in transarterial chemoembolization for liver tumor[J]. J Vasc Interv Radiol, 2017, 28: 1461-1466.
[18] Trissel LA. Avoiding common flaws in stability and compatibility studies of injectable drugs[J]. Am J Hosp Pharm, 1983, 40: 1159-1160.
[19]王振华. 利多卡因硬膜外麻醉意外法医毒物动力学研究(八)[D]. 太原:山西医科大学,2008.
[20]王天成,马 聪,肖煜东,等. 利多卡因-表柔比星-超液化碘油混合乳剂在肝癌TACE相关疼痛管理中的应用[J]. 介入放射学杂志,2020,29:366-371.
[21]李 军,裘敏剑,章士正. 肝癌经导管动脉栓塞化疗术中利多卡因碘油乳剂止痛效果评价[J]. 全科医学临床与教育,2011,9:431-432.
[22]陈丽霞,杨永明,魏锦萍,等. 利多卡因对丝裂霉素治疗肝癌H22荷瘤小鼠的增敏作用研究[J]. 肿瘤研究与临床,2019,31:84-87.
[23]曾志宏,王 奕,胡含明,等. 利多卡因-碘油乳剂在原发性肝癌介入栓塞治疗中的临床应用[J]. 中国中西医结合影像学杂志,2009,7:151-152.
[24]王小会,鲁 岩. 利多卡因碘油乳剂在经导管动脉内化疗栓塞中的止痛效果评价[J]. 肿瘤基础与临床,2011,24:224-246.
[25] Wang Y, Ou-Yang QG, Huang WL, et al. Investigation of the inhibitory effect of simvastatin on the metabolism of lidocaine both in vitro and in vivo[J]. Drug Des Devel Ther, 2020, 14:1739-1747.

相似文献/References:

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 WANG Tiancheng,MA Cong,XIAO Yudong,et al.The application of lidocaine-epirubicin-lipiodol emulsion in the management of TACE-related pain in HCC patients[J].journal interventional radiology,2020,29(01):366.

备注/Memo

备注/Memo:
(收稿日期:2021-09-06)
(本文编辑:新 宇)  
更新日期/Last Update: 2023-02-13