[1]王东东,彭金钊,李云芳,等.兔脊柱旁肌肉VX2种植瘤模型建立及生物学特性 [J].介入放射学杂志,2019,28(06):561-565.
 WANG Dongdong,PENG Jinzhao,LI Yunfang,et al.Establishment of rabbit VX2 tumor model in paraspinal muscle and its biological characteristics[J].journal interventional radiology,2019,28(06):561-565.
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兔脊柱旁肌肉VX2种植瘤模型建立及生物学特性
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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
28
期数:
2019年06期
页码:
561-565
栏目:
实验研究
出版日期:
2019-06-25

文章信息/Info

Title:
Establishment of rabbit VX2 tumor model in paraspinal muscle and its biological characteristics
作者:
王东东 彭金钊 李云芳 李晓光
Author(s):
WANG Dongdong PENG Jinzhao LI Yunfang LI Xiaoguang.
Peking Union Medical College & Chinese Academy of Medical Sciences, Center of Minimally- Invasive Therapies for Tumors of Beijing Hospital, National Center of Gerontology, Beijing 100061, China
关键词:
【关键词】 VX2 脊柱旁肌肉 动物模型 CT
文献标志码:
A
摘要:
【摘要】 目的 建立兔脊柱旁肌肉VX2种植瘤模型,观察其生物学特性。方法 CT导引下经皮穿刺将VX2肿瘤组织块种植于15只新西兰大白兔脊柱旁肌肉,构建肿瘤模型。种植术后第14、21、28、35天行CT平扫和增强扫描,随机选取1只荷瘤兔处死,作大体解剖及病理学检查。结果 15只实验兔脊柱旁肌肉VX2肿瘤组织块均成功种植,建模成功14只,成瘤率为93.3%。种植瘤CT平扫呈低密度结节灶,边缘模糊;CT增强扫描病变呈环状强化,边缘模糊,中心见低密度坏死区。种植瘤生长迅速,第28天与第21天肿瘤生长率(TGR)比较,差异有显著统计学意义(P=0.004);第35天与第28天TGR比较,差异有显著统计学意义(P=0.01)。种植术后第14~28天,瘤体大小为1.0~2.5 cm。种植瘤坏死率第14、21、28天间差异均有显著统计学意义(P<0.01),第28、35天间差异无统计学意义(P=0.243)。种植瘤病理组织学呈浸润性生长,内部粥糜样坏死;肿瘤细胞呈条索状或巢状弥漫分布,间质血管丰富;肿瘤细胞形态不规则,体积较大,细胞异型性明显并见较多核分裂像。第35天时,5只荷瘤兔CT增强扫描发现腹膜后淋巴结和肺转移,2只荷瘤兔脊髓受侵犯。结论 脊柱旁肌肉种植瘤恶性度高,生长速度快,成瘤时间短,瘤组织血供丰富,是一种较为理想的用于肿瘤局部治疗研究的实验模型。

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备注/Memo

备注/Memo:
(收稿日期:2018-07-09)
(本文编辑:边 佶)
更新日期/Last Update: 2019-06-11