[1]王 栋,宫卫东,黄 辞,等.MS-275对胃腺癌细胞抑制作用及其机制研究[J].介入放射学杂志,2015,(04):333-337.
 WANG Dong,GONG Wei dong,HUANG Ci,et al.Inhibitory effect of MS 275 on gastric glandular cancer cells and its possible mechanism [J].journal interventional radiology,2015,(04):333-337.
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MS-275对胃腺癌细胞抑制作用及其机制研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2015年04期
页码:
333-337
栏目:
实验研究
出版日期:
2015-04-25

文章信息/Info

Title:

Inhibitory effect of MS 275 on gastric glandular cancer cells and its possible mechanism

作者:
王 栋 宫卫东 黄 辞 李中华 刘振堂 吴智群
Author(s):
WANG Dong GONG Wei dong HUANG Ci LI Zhong hua LIU Zhen tang WU Zhi qun
Department of Interventional Radiology, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi Province 710038, China
关键词:
MS-275 胃腺癌 SGC 7901 线粒体凋亡 细胞周期阻滞
文献标志码:
A
摘要:
目的 采用分子靶向药物治疗胃癌是近年研究热点。探讨组蛋白去乙酰化酶抑制因子MS 275通过多种途径选择性杀伤人胃低分化腺癌细胞株SGC 7901的机制。方法 10~100 μmol/L 药物浓度梯度的MS 275分别与SGC 7901、人正常胃黏膜上皮细胞株GES 1共培养24 h后,采用WST 1法检测SGC 7901及GES 1细胞存活率,流式细胞仪检测分析SGC 7901细胞线粒体膜电位变化,Western blot、实时荧光定量聚合酶链反应(RTQ PCR)分别检测处理后胃癌细胞中p21、p27、p57、cyclin B1、cyclin D1基因及相应蛋白表达情况。结果 MS 275可使SGC 7901细胞存活率降低(P<0.05),其作用随药物浓度增大更明显,对GES 1细胞无显著影响;MS 275可降低SGC 7901细胞线粒体膜电位(P<0.05);MS 275显著提升p21、p27、p57基因及相应蛋白相对含量,同时降低cyclin B1、cyclin D1基因及其蛋白相对含量(P<0.05)。结论 MS 275可选择性杀伤胃腺癌细胞SGC 7901,这一作用可能是通过线粒体凋亡途径及调控细胞周期相关基因和蛋白表达实现的。

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备注/Memo

备注/Memo:
(收稿日期:2014-09-04)
(本文编辑:边 佶)
更新日期/Last Update: 2015-04-27