[1]王建波,杨建勇,陈伟,等.地塞米松对血管内支架介入术后再狭窄作用的实验研究[J].介入放射学杂志,2007,(03):194.
 WANG Jian-bo,YANG Jian-yong,CHEN Wei,et al.Experimental study on effect of dexamethasone to the in-stent restenosis after vascular intervention[J].journal interventional radiology,2007,(03):194.
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地塞米松对血管内支架介入术后再狭窄作用的实验研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2007年03期
页码:
194
栏目:
实验研究
出版日期:
2007-03-15

文章信息/Info

Title:
Experimental study on effect of dexamethasone to the in-stent restenosis after vascular intervention
作者:
王建波 杨建勇 陈伟 庄文权 张珑涓 李家平
Author(s):
WANG Jian-bo YANG Jian-yongCHEN WeiZHUANG Wen-quanZHANG Long-juanLI Jia-ping.
Department of Interventional Radiology,The6th People's Hospital Shanghai Jiaotong University Shanghai200233 China Corresponding author YANG Jian-young Laboratory of Surgery,The First Affiliated Hospital,SUN Yet-sen University,Guangzhou510080,China
关键词:
地塞米松血管平滑肌细胞细胞增殖
分类号:
R543.1
文献标志码:
A
摘要:
目的观察地塞米松对体外培养大鼠胸主动脉平滑肌细胞(SMC)增殖的作用,探讨其对血管内支架介入术后远期再狭窄的防治价值。方法组织贴块法体外原代培养大鼠胸主动脉SMC,取第6~10代作实验用,MTT法检测地塞米松不同浓度和不同作用时间对SMC增殖的效果,并用RT-PCR法检测不同浓度地塞米松作用下大鼠胸主动脉SMC增殖细胞核抗原(PCNA)mRNA的表达情况。结果①地塞米松呈剂量依赖性抑制体外培养的大鼠SMC增殖,不同时间和不同浓度对其抑制程度不同。各浓度组之间总A值变化经方差分析差异有显著性,F=36.02,P<0.001;两两比较较高浓度组10 -6 及10 -5 mol/L抑制率相似,A值无显著性差异,P=0.065;低浓度组10 -11 mol/L与对照组相比差异无显著性,P=0.567;余各两两比较差异均有显著性,P<0.001;②地塞米松以浓度依赖模式抑制大鼠SMC的PCNA mRNA表达。方差分析F=15.407,P<0.001。较低浓度组(10 -9 mol/L及10 -11 mol/L)与对照组相比,无显著性差异,P值分别为0.48及0.56。其余各组同对照组相比,差异有显著性,P<0.05。结论地塞米松呈剂量依赖性抑制大鼠SMC增殖,10 -7 mol/L浓度以上可起效,并能持续抑制96h以上,较低浓度地塞米松在作用时间延长时也有可能抑制SMC的增殖。

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2006-02-27
更新日期/Last Update: 2007-03-15