[1]余海洋,游德淑,顾晓诚,等.基于生物3D打印技术的芍药苷-海藻酸钠-明胶皮肤支架体外生物相容性研究[J].介入放射学杂志,2022,31(06):582-586.
YU Haiyang,YOU Deshu,GU Xiaocheng,et al.Bio-3D printing technique-based paeoniflorin-sodium alginate-gelatin skin scaffold: an experimental study of its biocompatibility in vitro[J].journal interventional radiology,2022,31(06):582-586.
点击复制
基于生物3D打印技术的芍药苷-海藻酸钠-明胶皮肤支架体外生物相容性研究()
《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]
- 卷:
-
31
- 期数:
-
2022年06
- 页码:
-
582-586
- 栏目:
-
非血管介入
- 出版日期:
-
2022-07-31
文章信息/Info
- Title:
-
Bio-3D printing technique-based paeoniflorin-sodium alginate-gelatin skin scaffold: an experimental study of its biocompatibility in vitro
- 作者:
-
余海洋; 游德淑; 顾晓诚; 梅峻豪; 秦立昊; 王 凯; 刘 纯; 王 云; 贾中芝
-
- Author(s):
-
YU Haiyang; YOU Deshu; GU Xiaocheng; MEI Junhao; QIN Lihao; WANG Kai; LIU Chun; WANG Yun; JIA Zhongzhi.
-
Department of Interventional and Vascular Surgery, Affiliated Changzhou Municipal No.2 People’s Hospital, Nanjing Medical University, Changzhou, Jiangsu Province 213003, China
-
- 关键词:
-
【关键词】 生物3D打印; 皮肤支架; 芍药苷; 海藻酸钠; 明胶; 生物相容性; 体外
- 文献标志码:
-
A
- 摘要:
-
【摘要】 目的 探讨采用生物3D打印技术制备的芍药苷-海藻酸钠(SA)-明胶皮肤支架的体外生物相容性。 方法 通过生物3D打印技术制备质量百分比分别为0、1、3、5、10%的芍药苷-SA-明胶皮肤支架。采用扫描电子显微镜(SEM)观察其表面形态结构,高效液相色谱仪检测芍药苷累积释放浓度。将大鼠皮肤成纤维细胞与其共培养3 d,行Live/Dead染色,观察支架表面细胞存活和生长状态,检测1、2、3 d时细胞增殖、羟脯氨酸和白细胞介素(IL)-6表达水平。 结果 SEM显示5组皮肤支架均呈多孔网状立体结构,孔隙均匀。3%组皮肤支架表面较其他4组光滑。药物累积释放量随着时间延长逐渐增加,10 %皮肤支架组在各时点的释放均高于其他4组。Live/Dead染色显示细胞在5组皮肤支架表面均能较好地黏附,生长良好,SEM显示3%组皮肤支架活细胞数多于其他4组。3%组皮肤支架的细胞增殖率、羟脯氨酸分泌水平显著高于其他4组(均P<0.05),IL-6表达水平显著低于0和1%组(P<0.01),但高于10%组(P<0.01)。 结论 3%芍药苷-SA-明胶皮肤支架实现了生物效应与细胞毒性的平衡,具有良好的生物相容性和胶原蛋白分泌效应,可有效抑制炎症反应发生,有望为糖尿病足溃疡创面修复提供一种可降解的生物皮肤支架。
参考文献/References:
[1] Aumiller WD, Dollahite HA. Pathogenesis and management of diabetic foot ulcers[J]. JAAPA, 2015, 28: 28- 34.
[2] Jneid J, Lavigne JP, Scola BL, et al. The diabetic foot microbiota: a review[J]. Human Microbiome J, 2017, 9: 1- 6.
[3] Hobizal KB, Wukich DK. Diabetic foot infections: current concept review[J]. Diabet Foot Ankle, 2012, 3: 1- 8.
[4] Wang P, Wang W, Shi Q, et al. Paeoniflorin ameliorates acute necrotizing pancreatitis and pancreatitisinduced acute renal injury[J]. Mol Med Rep, 2016, 14: 1123- 1131.
[5] Jia Z, He J. Paeoniflorin ameliorates rheumatoid arthritis in rat models through oxidative stress, inflammation and cyclooxygenase 2[J]. Exp Ther Med, 2016, 11: 655- 659.
[6] 张育贵,张淑娟,边甜甜,等. 芍药苷药理作用研究新进展[J].中草药, 2019, 50:3735- 3740.
[7] Pahlevanzadeh F, Mokhtari H, Bakhsheshi- Rad HR, et al. Recent trends in three- dimensional bioinks based on alginate for biomedical applications[J]. Materials(Basel), 2020, 13: 3980.
[8] Zhou G, Liu W, Zhang Y, et al. Application of three- dimensional printing in interventional medicine[J]. J Intervent Med, 2020, 3: 1- 16.
[9] 陈 川,周 耕,卢 川,等. 三维打印快速仿形技术在介入医学领域的应用价值[J]. 介入放射学杂志, 2016, 25:734- 737.
[10] Yang W, Lu J, Weng J, et al. Prevalence of diabetes among men and women in China[J]. N Engl J Med, 2010, 362: 1090- 1101.
[11] 胡晓晓,李茂全,姜金霞. 持续冲洗负压封闭引流联合光子治疗在糖尿病足溃疡的疗效及护理[J]. 介入放射学杂志, 2018, 27:672- 676.
[12] Eguchi K, Nagai R. Islet inflammation in type 2 diabetes and physiology[J]. J Clin Invest, 2017, 127: 14- 23.
[13] Abaci HE, Guo Z, Doucet Y, et al. Next generation human skin constructs as advanced tools for drug development[J]. Exp Biol Med(Maywood), 2017, 242: 1657- 1668.
[14] Mofazzal Jahromi MA, Sahandi Zangabad P, Moosavi Basri SM, et al. Nanomedicine and advanced technologies for burns: preventing infection and facilitating wound healing[J]. Adv Drug Deliv Rev, 2018, 123: 33- 64.
[15] 顾其胜,朱 彬. 海藻酸盐基生物医用材料[J]. 中国组织工程研究与临床康复, 2007, 11:5194- 5198.
[16] Su K, Wang C. Recent advances in the use of gelatin in biomedical research[J]. Biotechnol Lett, 2015, 37: 2139- 2145.
[17] 张 茜,卜德懿,田永鑫,等. 胶原纤维的酶解液中羟脯氨酸含量测定方法的优化[J]. 皮革科学与工程, 2019, 29:25- 30.
[18] 胡 康,张 伟. 胶原蛋白作为医用生物材料对缺损组织修复、再生及重建的作用与意义[J]. 中国组织工程研究, 2019, 23:317- 322.
[19] 陈 炜,赵 磊,牛素平,等.不同炎症因子对细菌性血流感染所致脓毒症患者的早期诊断价值[J].中华危重病急救医学,2014, 26:165- 170.
[20] Cao W, Zhang W, Liu J, et al. Paeoniflorin improves survival in LPS- challenged mice through the suppression of TNF- α and IL- 1β release and augmentation of IL- 10 production[J]. Int Immunophar- macol, 2011, 11: 172- 178.
[21] Liu S, Zhang Q, Yu J, et al. Absorbable thioether grafted hyaluronic acid nanofibrous hydrogel for synergistic modulation of inflammation microenvironment to accelerate chronic diabetic wound healing[J]. Adv Healthc Mater, 2020, 9: e2000198.
备注/Memo
- 备注/Memo:
-
(收稿日期:2021- 08- 13)
(本文编辑:边 佶)
更新日期/Last Update:
2022-07-29