[1]余海洋,游德淑,顾晓诚,等.基于生物3D打印技术的芍药苷-海藻酸钠-明胶皮肤支架体外生物相容性研究[J].介入放射学杂志,2022,31(06):582-586.
 YU Haiyang,YOU Deshu,GU Xiaocheng,et al.Bio-3D printing technique-based paeoniflorin-sodium alginate-gelatin skin scaffold: an experimental study of its biocompatibility in vitro[J].journal interventional radiology,2022,31(06):582-586.
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基于生物3D打印技术的芍药苷-海藻酸钠-明胶皮肤支架体外生物相容性研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
31
期数:
2022年06
页码:
582-586
栏目:
非血管介入
出版日期:
2022-07-31

文章信息/Info

Title:
Bio-3D printing technique-based paeoniflorin-sodium alginate-gelatin skin scaffold: an experimental study of its biocompatibility in vitro
作者:
余海洋 游德淑 顾晓诚 梅峻豪 秦立昊 王 凯 刘 纯 王 云 贾中芝
Author(s):
YU Haiyang YOU Deshu GU Xiaocheng MEI Junhao QIN Lihao WANG Kai LIU Chun WANG Yun JIA Zhongzhi.
Department of Interventional and Vascular Surgery, Affiliated Changzhou Municipal No.2 People’s Hospital, Nanjing Medical University, Changzhou, Jiangsu Province 213003, China
关键词:
【关键词】 生物3D打印 皮肤支架 芍药苷 海藻酸钠 明胶 生物相容性 体外
文献标志码:
A
摘要:
【摘要】 目的 探讨采用生物3D打印技术制备的芍药苷-海藻酸钠(SA)-明胶皮肤支架的体外生物相容性。 方法 通过生物3D打印技术制备质量百分比分别为0、1、3、5、10%的芍药苷-SA-明胶皮肤支架。采用扫描电子显微镜(SEM)观察其表面形态结构,高效液相色谱仪检测芍药苷累积释放浓度。将大鼠皮肤成纤维细胞与其共培养3 d,行Live/Dead染色,观察支架表面细胞存活和生长状态,检测1、2、3 d时细胞增殖、羟脯氨酸和白细胞介素(IL)-6表达水平。 结果 SEM显示5组皮肤支架均呈多孔网状立体结构,孔隙均匀。3%组皮肤支架表面较其他4组光滑。药物累积释放量随着时间延长逐渐增加,10 %皮肤支架组在各时点的释放均高于其他4组。Live/Dead染色显示细胞在5组皮肤支架表面均能较好地黏附,生长良好,SEM显示3%组皮肤支架活细胞数多于其他4组。3%组皮肤支架的细胞增殖率、羟脯氨酸分泌水平显著高于其他4组(均P<0.05),IL-6表达水平显著低于0和1%组(P<0.01),但高于10%组(P<0.01)。 结论 3%芍药苷-SA-明胶皮肤支架实现了生物效应与细胞毒性的平衡,具有良好的生物相容性和胶原蛋白分泌效应,可有效抑制炎症反应发生,有望为糖尿病足溃疡创面修复提供一种可降解的生物皮肤支架。

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备注/Memo

备注/Memo:
(收稿日期:2021- 08- 13)
(本文编辑:边 佶)
更新日期/Last Update: 2022-07-29