[1]何子聪,曹彦龙,李承志,等.DSA灌注成像在急性缺血-再灌注损伤兔模型中的应用[J].介入放射学杂志,2019,28(08):765-769.
 HE Zicong,CAO Yanlong,LI Chengzhi,et al.Application of DSA perfusion imaging in rabbit model with acute ischemia-reperfusion injury[J].journal interventional radiology,2019,28(08):765-769.
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DSA灌注成像在急性缺血-再灌注损伤兔模型中的应用()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
28
期数:
2019年08期
页码:
765-769
栏目:
实验研究
出版日期:
2019-08-25

文章信息/Info

Title:
Application of DSA perfusion imaging in rabbit model with acute ischemia-reperfusion injury
作者:
何子聪 曹彦龙 李承志 张 艳
Author(s):
HE Zicong CAO Yanlong LI Chengzhi ZHANG Yan.
Department of Interventional Radiology and Vascular Surgery, First Affiliated Hospital of Jinan University, Guangzhou, Guangdong Province 510630, China
关键词:
【关键词】 数字化三维血管造影 缺血- 再灌注损伤 DSA灌注成像
文献标志码:
A
摘要:
【摘要】 目的 探讨DSA灌注成像技术评估骨骼肌缺血- 再灌注损伤(IRI)的应用价值。方法 将30只新西兰大白兔随机分为IRI 0 h组、6 h组、12 h组、24 h组和对照组,每组各6只。IRI各组分别在右下肢缺血3 h恢复血流灌注后0 h、6 h、12 h、24 h接受双下肢动脉3D DSA造影,对照组不予特殊处理即行双下肢动脉3D造影,同时检测血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平变化。用DSA灌注成像后处理软件Syngo DynaPBV分析造影图像,计算出双下肢灌注参数之血流容积(BV)及其两者比值之相对血流容积(rBV),将各时点测得的rBV与实验室指标作相关性分析。结果 与对照组相比,DSA灌注参数rBV在IRI 6 h组、12 h组、24 h组间差异有统计学意义(P<0.05);IRI各组间比较,rBV随再灌注时间延长逐渐降低(F=5.816,P<0.05)。Pearson相关性分析显示,IRI组各时点rBV值与实验室检查指标CK(r=- 0.91,P<0.05)、LDH(r=- 0.90,P<0.05)、MDA(r=- 0.85,P<0.05)呈负相关,与SOD(r=0.74,P<0.05)呈正相关。结论 术中应用DSA灌注成像技术评估肢体IRI安全可行,其灌注参数与实验室指标间有较好相关性,可实时评估术中骨骼肌IRI程度。

参考文献/References:

[1] Wang WZ, Baynosa RC, Zamboni WA. Therapeutic interventions against reperfusion injury in skeletal muscle[J]. J Surg Res, 2011, 171: 175- 182.
[2] Huang T, Wang W, Tu C, et al., Hydrogen- rich saline attenuates ischemia- reperfusion injury in skeletal muscle[J]. J Surg Res, 2015, 194: 471- 480.
[3] Suzuki M, Tanaka R, Yoshioka K, et al., Subtraction CT angiography for the diagnosis of iliac arterial steno- occlusive disease[J]. Jpn J Radiol, 2016, 34: 194- 202.
[4] Kim AH, Shevitz AJ, Morrow KL, et al., Characterizing tissue perfusion after lower extremity intervention using two- dimensional color- coded digital subtraction angiography[J]. J Vasc Surg, 2017, 66: 1464- 1472.
[5] Hinrichs JB, Murray T, Akin M, et al., Evaluation of a novel 2D perfusion angiography technique independent of pump injections for assessment of interventional treatment of peripheral vascular disease[J]. Int J Cardiovasc Imaging, 2017, 33:295- 301.
[6] Shakur SF, Brunozzi D, Hussein AE, et al., Validation of cerebral arteriovenous malformation hemodynamics assessed by DSA using quantitative magnetic resonance angiography: preliminary study[J]. J Neurointerv Surg, 2018, 10: 156- 161.
[7] Yang M, Wu J, Ma L, et al., The value of syngo dynapbv neuro during neuro- interventional hypotensive balloon occlusion test[J]. Clin Neuroradiol, 2015, 25: 387- 395.
[8] Bley T, Strother CM, Pulfer K, et al. C- arm CT measurement of cerebral blood volume in ischemic stroke: an experimental study in canines[J]. AJNR Am J Neuroradiol, 2010, 31: 536- 540.
[9] Fujimoto M, Itokawa H, Moriya M, et al. Evaluation of cerebral hyperperfusion after carotid artery stenting using C- arm CT mea-surements of cerebral blood volume[J]. Clin Neuroradiol, 2018, 28: 253- 260.
[10] Wen LL, Zhang X, Zhang QR, et al. Flat- detector computed tomography PBV map in the evaluation of presurgical embolization for hypervascular brain tumors[J]. J Neurointerv Surg, 2017, 9: 1139- 1144.
[11] Zhuang ZG, Zhang XB, Han JF, et al. Hepatic blood volume imaging with the use of flat- detector CT perfusion in the angiography suite: comparison with results of conventional multislice CT perfusion[J]. J Vasc Interv Radiol, 2014, 25: 739- 746.
[12] Pereira PL, Kruger K, Hohenstein E, et al. Intraprocedural 3D perfusion measurement during chemoembolisation with doxorubicin- eluting beads in liver metastases of malignant melanoma[J]. Eur Radiol, 2018, 28: 1456- 1464.
[13] Carden DL, Granger DN. Pathophysiology of ischaemia- reperfusion injury[J]. J Pathol, 2000, 190: 255- 266.
[14] Carmo- Araujo EM, Dal- Pai- Silva M, Dal- Pai V, et al. Ischaemia and reperfusion effects on skeletal muscle tissue: morphological and histochemical studies[J]. Int J Exp Pathol, 2007, 88: 147- 154.
[15] 范 里,刘世清. 骨骼肌缺血再灌注损伤后的血液流变性及微循环变化[J]. 微循环学杂志, 1999, 9: 13- 15.
[16] Bosco G, Yang ZJ, Nandi J, et al. Effects of hyperbaric oxygen on glucose, lactate, glycerol and anti- oxidant enzymes in the skeletal muscle of rats during ischaemia and reperfusion[J]. Clin Exp Pharmacol Physiol, 2007, 34: 70- 76.

备注/Memo

备注/Memo:
(收稿日期:2018- 10- 30)
(本文编辑:边 佶)
更新日期/Last Update: 2019-08-21