[1]阚科佳,齐昊喆,杨硕菲,等.舒洛地特对氧化型低密度脂蛋白诱导人脐静脉内皮细胞损伤的保护作用及其机制研究 [J].介入放射学杂志,2017,(06):539-543.
 KAN Kejia,QI Haozhe,YANG Shuofei,et al.Protective effect of sulodexide on ox- LDL induced damage to human umbilical vein endothelial cells and its mechanism [J].journal interventional radiology,2017,(06):539-543.
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舒洛地特对氧化型低密度脂蛋白诱导人脐静脉内皮细胞损伤的保护作用及其机制研究


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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2017年06期
页码:
539-543
栏目:
实验研究
出版日期:
2017-06-25

文章信息/Info

Title:
Protective effect of sulodexide on ox- LDL induced damage to human umbilical vein endothelial cells and its mechanism
作者:
阚科佳 齐昊喆 杨硕菲 倪其泓 郭相江 陈佳佺 张 岚
Author(s):
KAN Kejia QI Haozhe YANG Shuofei Ni Qihong GUO Xiangjiang CHEN Jiaquan ZHANG Lan
Department of Vascular Surgery Affiliated Renji Hospital School of Medicine Shanghai Jiaotong University Shanghai 200127 China
关键词:
【关键词】 舒洛地特 氧化型低密度脂蛋白 人脐静脉内皮细胞 小凹蛋白- 1 内皮型一氧化氮合酶
文献标志码:
A
摘要:
【摘要】 目的 研究舒洛地特(SDX)对氧化型低密度脂蛋白(ox- LDL)诱导人脐静脉内皮细胞(HUVEC)损伤的保护作用及其机制。方法 CCK- 8法确定ox- LDL干预HUVEC剂量及SDX药物浓度,活性氧(ROS)检测试剂盒验证SDX对HUVEC的保护作用。实时荧光定量-聚合酶链反应(RT- PCR)检测内皮型一氧化氮合酶(eNOS)、小凹蛋白(caveolin)- 1 mRNA表达,免疫印迹试验检测eNOS、caveolin- 1蛋白表达。Transwell试验检测HUVEC迁移能力,免疫印迹试验检测抗磷酸化eNOS(p- eNOS)蛋白表达。结果 100 μg/ml ox- LDL刺激下, HUVEC细胞活力显著下降(P<0.01),加入0.125 LRU/ml SDX后细胞活力明显改善(P<0.01),ROS产生降低(P<0.01)。SDX可下调ox- LDL损伤HUVEC后caveolin- 1 mRNA和蛋白表达(P<0.05),上调eNOS mRNA和蛋白、p- eNOS蛋白表达(P<0.05),明显改善受损HUVEC迁移能力(P<0.01)。结论 SDX通过调控caveolin- 1/eNOS信号通路改善受损HUVEC细胞迁移能力,从而保护内皮细胞。

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备注/Memo

备注/Memo:
(收稿日期:2017-03-23)
(本文编辑:边 佶)
更新日期/Last Update: 2017-06-13