[1]宋伦圭.16S rRNA基因测序技术在肝脓疡细菌鉴定中的作用[J].介入放射学杂志,2014,(10):906-912.
Yun Gyu Song,M.D.,Sang Gun Shim,et al.Usefulness of 16S rRNA Gene Sequencing for Identification of Bacteria from Pyogenic Liver Abscess [J].journal interventional radiology,2014,(10):906-912.
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16S rRNA基因测序技术在肝脓疡细菌鉴定中的作用 ()
《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]
- 卷:
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- 期数:
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2014年10期
- 页码:
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906-912
- 栏目:
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临床研究
- 出版日期:
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2014-10-25
文章信息/Info
- Title:
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Usefulness of 16S rRNA Gene Sequencing for Identification of Bacteria from Pyogenic Liver Abscess
- 作者:
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宋伦圭
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- Author(s):
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Yun Gyu Song; M.D.; Sang Gun Shim; M.D.; Ph.D.; Kwang Min Kim; M.D.; Dae Soo Kim; Ph.D.; Sang Haeng Choi; Ph.D.; Jae Young Song; Ph.D.; Kon Ho Lee; Ph.D.; Hyung Lyun Kang; Ph.D.; Seung Chul Baik; M.D.; Ph.D.; Woo Kon Lee; V.M.D.; Ph.D.; Myung Je Cho; V.M.D.; Ph.D.; Kwang Ho Rhee; M.D.; Ph.D.; Dong Hae Lee.
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Department of Radiology; Department of Internal Medicine, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon, Korea; Human Derived Material Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea; Unitech Science Co., Ltd., Daejeon, Korea; Department of Microbiology, Gyeongsang National University College of Medicine, Jinju, Korea
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- 关键词:
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【关键词】 肝脓疡; 脓肿培养; 宏基因组学; 16S rRNA基因测序
- 文献标志码:
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A
- 摘要:
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【摘要】 目的 评价16S核糖体RNA(rRNA)基因测序在肝脓疡中细菌鉴定中的应用价值。方法2012年1月—2013年12月间共20例肝脓疡行经皮置管引流的患者,分别行脓液培养,血培养和16S rRNA基因测序。利用454 GS Junior System对脓液基因组DNA行PCR和16S rRNA基因测序。脓液培养,血液培养和16S rRNA 基因测序结果进行分别评价。结果 脓液和血液培养阳性的患者分别是9例(45%)和4例(20%)。16S rRNA基因测序细菌鉴定率为90%,明显高于传统的培养方法。结论 16S rRNA基因测序方法较传统的培养方法能更准确和有效对肝脓疡进行细菌鉴定。
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备注/Memo
- 备注/Memo:
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(Recived:2014-07-15)
(Editor:yuanzheng)
更新日期/Last Update:
2014-10-23