[1]魏黎明,付银鑫,朱悦琦,等.糖尿病大鼠骨髓来源内皮祖细胞体外生物学活性研究[J].介入放射学杂志,2014,(06):511-515.
　WEI Li- ming,FU Yin- xing,ZHU Yue- qi,et al. Bone marrow- derived endothelial progenitor cells in diabetic rats： study of biological activity in vitro[J].journal interventional radiology,2014,(06):511-515.
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糖尿病大鼠骨髓来源内皮祖细胞体外生物学活性研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2014年06期
页码:
511-515
栏目:
实验研究
出版日期:
2014-07-05

文章信息/Info

Title:
 Bone marrow- derived endothelial progenitor cells in diabetic rats： study of biological activity in vitro
作者:
 魏黎明;  付银鑫;  朱悦琦;  鲁海涛;  王 珏;  程永德;  李晓聪;  汪 泱;  赵俊功
Author(s):
 WEI Li- ming;  FU Yin- xing;  ZHU Yue- qi;  LU Hai- tao;  WANG Jue;  CHENG Yong- de;  LI Xiao- cong;  WANG Yang;  ZHAO Jun- gong.
 Department of Diagnostic and Interventional Radiology， Affiliated Sixth People’s Hospital， Shanghai Jiaotong University， Shanghai 200233， China
关键词:
 ; 【关键词】 糖尿病;  内皮祖细胞;  生物活性
文献标志码:
A
摘要:
 【摘要】 目的 探讨糖尿病大鼠骨髓来源内皮祖细胞与正常大鼠在数量及生物学活性方面的差异。方法 雄性SD大鼠按60 mg/kg腹腔快速注射1％ STZ的枸椽酸缓冲液建立糖尿病动物模型。密度梯度法分离糖尿病大鼠和正常大鼠骨髓单个核细胞，用EGMTM- 2MV BulletKitTM培养基进行诱导培养，观察细胞形态学特征，流式细胞仪检测细胞表面抗原，摄取Dil- Ac- LDL 与结合 FITC- UEA- 1 双荧光鉴定细胞及检测细胞比例，CCK- 8、Transwell小盒等检测细胞增殖、黏附及迁移能力。结果 内皮祖细胞在体外培养时呈梭形，贴壁生长，均可表达CD34、CD133和VEGFR- 2，且能摄取Dil- Ac- LDL并结合FITC- UEA- 1，证明所培养细胞为内皮祖细胞。糖尿病大鼠骨髓来源的内皮祖细胞数目与正常大鼠相比无明显差异，但其黏附能力明显低于正常大鼠，增殖能力、黏附及迁移能力均降低。结论 与正常大鼠相比，糖尿病大鼠骨髓来源内皮祖细胞数量无明显差异，但生物学活性降低，主要表现在增殖能力、黏附及迁移能力均减弱。

参考文献/References:

 ［1］ Chen J， Chen J， Chen S， et al. Transfusion of CXCR4- primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice［J］. PLoS One， 2012， 7： e50105.
［2］ Tatsumi T， Matsubara H. Therapeutic angiogenesis for peripheral arterial disease and ischemic heart disease by autologous bone marrow cells implantation［J］. Nihon Rinsho， 2006， 64： 2126 - 2134.
［3］ Zhang W， Yan H. Dysfunction of circulating endothelial progenitor cells in type 1 diabetic rats with diabetic retinopathy［J］. Graefes Arch Clin Exp Ophthalmol， 2013， 251： 1123 - 1131.
［4］ Pendyala LK， Yin X， Li J， et al. The first- generation drug- eluting stents and coronary endothelial dysfunction［J］. JACC Cardiovasc Interv， 2009， 2： 1169 - 1177.
［5］ Murayama T， Tepper OM， Silver M， et al. Determination of bone marrow- derived endothelial progenitor cell significance in angiogenic growth factor- induced neovascularization in vivo［J］. Exp Hematol， 2002， 30： 967 - 972.
［6］ 肖 亮， 申 景， 黄德生， 等． 糖尿病下肢动脉阻塞性病变介入治疗疗效分析［J］． 介入放射学杂志， 2011， 20： 218 - 223．
［7］ Massa M， Rosti V， Ferrario M， et al. Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction［J］. Blood， 2005， 105： 199 - 206.
［8］ Sukpat S， Isarasena N， Wongphoom J， et al. Vasculoprotective effects of combined endothelial progenitor cells and mesenchymal stem cells in diabetic wound care： their potential role in decreasing wound- oxidative stress［J］. Biomed Res Int， 2013， 2013： 459196， doi： 10.1155/2013/459196.
［9］ Liu J， Zhang FF， Li L， et al. ClC- 3 deficiency prevents apoptosis induced by angiotensin Ⅱ in endothelial progenitor cells via inhibition of NADPH oxidase［J］. Apoptosis， 2013， 18： 1262 - 1273.
［10］ Aicher A， Heeschen C， Mildner- Rihm C， et al. Essential role of endothelial nitric oxide synthase for mobilization of stem and progenitor cells［J］. Nat Med， 2003， 9： 1370 - 1376.
［11］ Hasegawa T， Kosaki A， Shimizu K， et al. Amelioration of diabetic peripheral neuropathy by implantation of hematopoietic mononuclear cells in streptozotocin- induced diabetic rats［J］. Exp Neurol， 2006， 199： 274 - 280.
［12］ Rustemeyer P， Wittkowski W， Jurk K， et al. Optimized flow cytometric analysis of endothelial progenitor cells in peripheral blood［J］. J Immunoassay Immunochem， 2006， 27： 77 - 88.
［13］ Kim KA， Shin YJ， Kim JH， et al. Dysfunction of endothelial progenitor cells under diabetic conditions and its underlying mechanisms［J］. Arch Pharm Res， 2012， 35： 223 - 234.

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 （收稿日期：２０１3-11-０1）
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