[1]李红,徐克,蒋旭远.对兔移植性肝脏肿瘤模型生存素表达调控的实验研究[J].介入放射学杂志,2009,(05):382.
 LI Hong,XU Ke,JIANG Xu-yuan.The regulation of As2O3on the expression of apoptosis inhibitory protein:an experimental study in rabbits with implanted liver tumor[J].journal interventional radiology,2009,(05):382.
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对兔移植性肝脏肿瘤模型生存素表达调控的实验研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2009年05期
页码:
382
栏目:
实验研究
出版日期:
2009-05-15

文章信息/Info

Title:
The regulation of As 2 O 3 on the expression of apoptosis inhibitory protein:an experimental study in rabbits with implanted liver tumor
作者:
李红徐克蒋旭远
110001沈阳中国医科大学附属第一医院放射科
Author(s):
LI HongXU KeJIANG Xu-yuan.
Department of Radiology,the First Affiliated Hospital of China Medical University,Shenyang110001,China
关键词:
三氧化二砷肝脏肿瘤凋亡抑制生存素蛋白
分类号:
R73-3
文献标志码:
B
摘要:
目的探讨As 2 O 3 在肝动脉化疗栓塞(TACE)中诱导恶性肿瘤细胞凋亡作用与生存素之间的相关性。方法将肝脏左、右叶分别移植VX2肿瘤模型的16只日本大耳白兔,随机平均分成2组,移植瘤术后3周,经肝动脉插管分别给予超液化碘油(UFLP)1ml加As 2 O 3 2mg(实验组)和UFLP1ml(对照组)。给药3周后,所有动物均处死,分别取得肿瘤组织、瘤旁组织和正常肝脏组织进行末端脱氧核苷酸转移酶-生物素dUTP切口末端标记法(TUNEL)染色观察凋亡肿瘤细胞,免疫组化染色检测生存素蛋白的表达。结果实验组肿瘤组织中有大量呈黄色的凋亡细胞,癌旁组织及正常组织中未观察到凋亡细胞;对照组肿瘤组织、癌旁组织及正常组织均未发现细胞核内有黄色颗粒的凋亡细胞。对照组肿瘤组织生存素蛋白表达率为100%(16/16),其中强阳性12例,弱阳性4例,癌旁及正常组织中生存素蛋白表达率为0。实验组肿瘤组织生存素蛋白表达为37.5%(6/16),其中强阳性2例,弱阳性4例,癌旁及正常组织中生存素蛋白表达率同样为0。两组肿瘤组织中生存素蛋白表达率差异有统计学意义(P<0.05)。同时,两组癌组织与癌旁和正常组织中生存素蛋白表达率差异有统计学意义(P<0.01)。结论As 2 O 3 通过抑制肿瘤细胞内生存素蛋白表达促进肿瘤细胞凋亡。

参考文献/References:

[1]Hossain Z,Hosokawa M,Takahashi K.Growth inhibition and induction of apoptosis of colon cancer cell lines by applying marine phospholipid[J].Nutr Cancer,2009,61:123-130.
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备注/Memo

备注/Memo:
收稿日期:2008-12-01
更新日期/Last Update: 2009-05-15