[1]李永东,赵俊功,李明华,等.急性血栓性大脑中动脉栓塞脑缺血模型的建立[J].介入放射学杂志,2008,(02):122.
 LI Yongdong,ZHAO Jungong,LI Minghua,et al.Establishment of an acute focal cerebral ischemic model of middle cerebral artery occlusion with thrombus[J].journal interventional radiology,2008,(02):122.
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急性血栓性大脑中动脉栓塞脑缺血模型的建立()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2008年02期
页码:
122
栏目:
实验研究
出版日期:
2008-02-15

文章信息/Info

Title:
Establishment of an acute focal cerebral ischemic model of middle cerebral artery occlusion with thrombus
作者:
李永东赵俊功李明华
200233上海交通大学附属第六人民医院介入影像科
Author(s):
LI Yong-dongZHAO Jun-gongLI Ming-huaYOU Xiao-fangCHENG Ying-sheng.
The Sixth Affiliated People's Hospital,Shanghai Jiao-tong University,Shanghai200233,China
关键词:
脑缺血大脑中动脉栓塞磁共振大鼠
分类号:
R743.33
文献标志码:
A
摘要:
目的评价急性血栓性大鼠大脑中动脉栓塞(MCAO)脑缺血造模的可行性,旨在提高模型的可重复性和可控制性。方法健康雄性成年SD大鼠60只,体重300~450g,随机分为3组:大栓子组(栓子长1.2~1.5mm,15只)、中等栓子组(0.8~1.0mm,30只)和小栓子组(0.5~0.6mm,15只)。取同系大鼠的股动脉血0.6ml 与0.15ml 凝血酶溶液混匀后,注入微导管内制备成线样血栓。将切好的栓子经大鼠左侧颈内动脉注入,建立MCAO模型。使用GE Signa1.5T超导成像仪,3英寸环形表面线圈行大鼠脑MRI 检查,并将检查结果与病理结果对照。结果小栓子组15只,9只发现脑梗死灶(60%),中等栓子组和大栓子组所有大鼠均出现脑梗死灶,小栓子组与另2组比较差异有统计学意义(P<0.05)。中等栓子组脑梗死灶均位于同侧大脑半球,局限于左侧顶叶皮质、皮层下及基底节的占93.3%(28/30)。小栓子组9只,在24h或死亡时的平均脑梗死体积占同侧大脑半球的(14.41±8.72)%,中等栓子组30只占(48.29±18.57)%,大栓子组15只占(73.68±18.29)%。3组之间脑梗死体积比较差异有统计学意义(F=33.171,P<0.01)。小栓子组9只,平均生存时间(301.1±23.02)h;中等栓子组30只,平均生存时间(277.43±20.27)h;大栓子组15只,平均生存时间(59.93±25.03)h。大栓子组与另2组之间生存时间比较差异有统计学意义(F=24.676,P<0.01),而中等栓子组的生存时间与小栓子组比较差异无统计学意义(P>0.05)。中等栓子组脑梗死区相对脑血流容量(rCBV)在3~18h内比较差异无统计学意义(F=1.578,P>0.05)。结论经过改良后,中等栓子建立的大鼠MCAO模型脑梗死体积适中、存活率高、脑梗死部位恒定而rCBV持续降低,具有良好的可重复性和可控性。

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备注/Memo

备注/Memo:
收稿日期:2007-05-31
基金项目:国家自然科学基金资助(编号:30470506)
更新日期/Last Update: 2008-02-15