[1]陈胜利,全毅,黄子诚,等.Lp-THAE诱导兔VX２肝癌细胞凋亡[J].介入放射学杂志,2007,(06):406.
　CHEN Sheng-li,QUAN Yi,HUANG Zi-chen,et al.LP-THAE induced tumor cell apoptosis of rabbit VX２liver carcinoma[J].journal interventional radiology,2007,(06):406.
点击复制

Lp-THAE 诱导兔 VX２ 肝癌细胞凋亡()

PDF下载中关闭

分享到：

《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
期数:
2007年06期
页码:
406
栏目:
实验研究
出版日期:
2007-06-15

文章信息/Info

Title:
LP-THAE induced tumor cell apoptosis of rabbit VX２liver carcinoma
作者:
陈胜利; 全毅; 黄子诚; 等
５１０１８０广州市第一人民医院影像中心
Author(s):
CHEN Sheng-li; QUAN Yi; HUANG Zi-chen; CHEN Guo-dong; ZU Dong-liang．
The First Municipal People's Hospital of Guangzhou，Guangzhou５１０１８０，China
关键词:
凋亡; 肝癌; VX２肿瘤; 兔; 肝动脉; 化疗栓塞
分类号:
R７３５.７
文献标志码:
B
摘要:
目的研究Lp-THAE诱导兔肝VX２肿瘤细胞凋亡。方法２７只新西兰大白兔种植性VX２肝癌，均分成Lp-THAE组、THAI组和对照组，处理后２～５d处死动物，在肿瘤中央部位、外周部位和非瘤肝区正常组织取材。采用HE染色光镜观察典型凋亡细胞、计算凋亡指数，FITC-AnnexinV／PI双染色双变量流式细胞术分析凋亡细胞百分率。结果THAE组肿瘤中央与外周部位的凋亡指数分别为（１７．７６９±２．４１７）％，（４．１２９±１．１７２）％，P＜０．０１，凋亡细胞百分率分别为（１６．４８３±１．４０４）％，（９．４７８±０．９６４）％，P＜０．０１，坏死细胞百分率分别为（４３．５５９±５．０５３）％，（３３．４６０±１．８４０）％，P＝０．０９３。凋亡细胞与坏死细胞百分率总和分别为（６０．０４２±１３．９７９）％，（４２．９３８±８．９７９）％，P＜０．０１。THAE组的肿瘤细胞凋亡指数、凋亡细胞百分率和坏死细胞百分率都显著大于THAI组和对照组（P＜０．０１），肿瘤中央部位的细胞凋亡指数、凋亡细胞百分率都显著大于肿瘤外周部位（P＜０．０１）。结论Lp-THAE诱导肿瘤细胞凋亡和引起肿瘤细胞坏死是其作用机制的两种形式。

参考文献/References:

［１］兰春慧，房殿春，樊丽琳，等．介入治疗对兔VX２肝癌细胞增殖和凋亡的影响［J］．临床消化病杂志，２００５，１７：２０３-２０５．
［２］于继徐．TACE对肝癌细胞凋亡和增殖的影响［J］．中国医学影像技术，２００３，１９：１１５７-１１５９．
［３］Yoon CJ，Chung JW，Park JH，et al．Transcatheter arterial chemoembolization with paclitaxel-lipiodol solution in rabbitVX２liver tumor［J］．Radiology，２００３，２２９：１２６-１３１．
［４］张峰，田建明，康德强，等．不同浓度乙醇消融VX２兔移植性肝肿瘤的实验研究［J］．介入放射学杂志，２００５，１４：６２９-６３３．
［５］赵华栋，包国强，马庆久，等．射频消融诱导兔VX２肝脏移植瘤细胞凋亡［J］．消化外科，２００４，３：３６６-３７０．
［６］Formigli L，Conti A，Lippi D．″Falling leaves″：a survey of the history of apoptosis［J］．Minerva Med，２００４，９５：１５９-１６４．
［７］Van Cruchten S，Van Den Broeck W．Morphological and bioche-mical aspects of apoptosis，oncosis and necrosis［J］．Anat Histol Embryol，２００２，３１：２１４-２２３．
［８］郑骏年，谢叔良，陈家存，等．流式细胞术定量检测细胞凋亡３种方法的比较研究［J］．中国免疫学杂志，１９９９，１５：４６７-４６９．

相似文献/References:

[1]曹海利,孟巍,白彬.肝癌基因治疗研究新进展[J].介入放射学杂志,2008,(05):375.
　CAO Haili,MENG Wei,BAI Bin.New progress in research of gene therapy for hepatocellular carcinoma[J].journal interventional radiology,2008,(06):375.
[2]陆骊工,胡宝山,李勇,等.中晚期原发性肝癌合并症的介入治疗研究[J].介入放射学杂志,2008,(07):514.
　LU Ligong,HU Baoshan,LI Yong,et al.Study of interventional therapy for complications in advanced primary hepatocellular carcinoma[J].journal interventional radiology,2008,(06):514.
[3]杨伟洪,刘鹏程,梁珊瑚,等.多层螺旋CT血管造影在判断肝癌寄生供血中的价值[J].介入放射学杂志,2008,(09):654.
　YANG Weihong,LIU Pengchen g,LIANG Shanhu,et al.Evaluation of MSCTA for parasitic blood supply in hepatic carcinoma[J].journal interventional radiology,2008,(06):654.
[4]张茵,周兵,许秀芳,等.肝动脉化疗栓塞治疗原发性肝癌存活２０年一例[J].介入放射学杂志,2008,(09):684.
　ZHANG Yin,ZHOU Bing,XU Xiufang,et al.Survival more than２０years after transarterial chemical embolization for hepatocellular carcinoma：A case report[J].journal interventional radiology,2008,(06):684.
[5]关键,胡道予,卢凌,等.经动脉灌注５－FU缓释微球治疗兔VX２肝肿瘤[J].介入放射学杂志,2008,(11):799.
　GUAN Jian,HU Daoyu,LU Ling,et al.Experimental study of transarterial infusion with５－FU sustained－release microspheres for rabbit hepatic VX２tumors[J].journal interventional radiology,2008,(06):799.
[6]官泳松,贺庆.肝癌介入治疗中应用p５３的几个要点[J].介入放射学杂志,2008,(11):761.
　GUAN Yongsong,HE Qing.Essentials in clinical application of p５３for tumors intervention－example of liver cancer[J].journal interventional radiology,2008,(06):761.
[7]肖运平,肖恩华.介入治疗在防治肝癌术后复发中的作用及进展[J].介入放射学杂志,2008,(11):831.
　XIAO Yunping,XIAO Enhua.The role and progress of interventional therapy in the prevention and treatment of postoperative hepatocellular carcinoma recurrence[J].journal interventional radiology,2008,(06):831.
[8]史震山,陈自谦,杨熙章,等.原发性大肝癌患者TACE后S－腺苷蛋氨酸的护肝作用[J].介入放射学杂志,2008,(11):790.
　SHI Zhenshan,CHEN Ziqian,YANG Xizhang,et al.The clinical study on the liver－protection of S－adenosy－methionine in large hepatocellular carcinoma patients after TACE[J].journal interventional radiology,2008,(06):790.
[9]李亚洲,宫卫东,张瑞,等.组蛋白去乙酰化酶抑制剂诱导人胃癌细胞SGC－７９０１凋亡及其机制[J].介入放射学杂志,2010,(03):220.
　mechanism LI Yazhou,GONG Weidong,ZHAN Rui,et al.TSA－induced apoptosis of gastric carcinoma SGC－７９０１cell and its mechanism[J].journal interventional radiology,2010,(06):220.
[10]王耀普,杨康健,赵思源,等.兔肝癌模型的改良接种及其DSA影像分析[J].介入放射学杂志,2010,(03):214.
　WANG Yaopu,YANG Kangjian,ZHAO Siyuan,et al.The modified inoculation method for the preparation of rabbit VX２hepatic carcinoma model and DSA imagining observation[J].journal interventional radiology,2010,(06):214.

备注/Memo

备注/Memo:
收稿日期：２００６-０９-２９
基金项目：广东省医学科研基金项目（A２００３６２７）
更新日期/Last Update: 2007-06-15