[1]罗 荣,王凌霄,刘 航,等.臭氧化0.9%氯化钠溶液瘤内注射对兔VX2肿瘤低氧诱导因子-1α、血管内皮细胞生长因子表达及微血管密度形成的影响[J].介入放射学杂志,2020,29(09):921-925.
 LUO Rong,WANG Lingxiao,LIU Hang,et al.The effect of intratumoral injection of ozonated saline on hypoxia-inducible factor-1α, expression of vascular endothelial growth factor and microvessel density in rabbit VX2 tumor model[J].journal interventional radiology,2020,29(09):921-925.
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臭氧化0.9%氯化钠溶液瘤内注射对兔VX2肿瘤低氧诱导因子-1α、血管内皮细胞生长因子表达及微血管密度形成的影响()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
29
期数:
2020年09
页码:
921-925
栏目:
实验研究
出版日期:
2020-09-25

文章信息/Info

Title:
The effect of intratumoral injection of ozonated saline on hypoxia-inducible factor-1α, expression of vascular endothelial growth factor and microvessel density in rabbit VX2 tumor model
作者:
罗 荣 王凌霄 刘 航 杨朝爱 江 旭 杨继金
Author(s):
LUO Rong WANG Lingxiao LIU Hang YANG Chaoai JIANG Xu YANG Jijin.
Department of Interventional Radiology, Affiliated Changhai Hospital, Naval Medical University, Shanghai 200433, China
关键词:
【关键词】 VX2肿瘤 臭氧 低氧诱导因子-1α 血管内皮细胞生长因子 微血管密度
文献标志码:
A
摘要:
【摘要】 目的 探讨臭氧化0.9%氯化钠溶液瘤内注射对兔VX2肿瘤组织内低氧诱导因子(HIF)-1α、血管内皮细胞生长因子(VEGF)表达及微血管密度(MVD)形成的影响。方法 制备新西兰大白兔VX2肿瘤模型72只,随机分为4组,各18只。超声导引下分别予以瘤内单次注射0.9%氯化钠溶液(A组)、单次注射臭氧化0.9%氯化钠溶液(B组)、2次注射臭氧化0.9%氯化钠溶液(1次/d,连续2 d,C组),3次注射臭氧化0.9%氯化钠溶液(1次/d,连续3 d,D组),剂量均为2倍肿瘤体积。术后第4、8、12天各组分别随机处死6只兔并切取肿瘤,采用免疫组化法检测肿瘤组织内HIF-1α、VEGF表达及MVD形成。采用方差分析进行统计学分析。结果 术后第4、8天B组HIF-1α表达低于A组,第12天略高于A组;术后C、D组HIF-1α表达均高于A组,C组更明显,第12天C组与A、B、D组间差异均有统计学意义(P<0.05)。术后第4天B、C组VEGF表达稍低于A组,其余时间点及D组VEGF表达均高于A组,且在第8天C、D组与A组间差异均有统计学意义(P<0.05)。与A组相比,术后第4、8天B组瘤组织内MVD形成减少,术后第12天略增高;C组MVD形成在各时间点均高于A、B、D组,第4天D组MVD形成高于A组,第8、12天低于A组。结论 臭氧化0.9%氯化钠溶液瘤内单次注射对肿瘤组织内HIF-1α、VEGF表达及MVD形成无明显影响,但多次注射可能导致HIF-1α、VEGF高表达,MVD形成呈增加趋势。

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备注/Memo

备注/Memo:
(收稿日期:2019- 06- 01)
(本文编辑:边 佶)
更新日期/Last Update: 2020-09-15