[1]杨 鹏,何 洋,李 成,等.微小核糖核酸-7调控表皮细胞生长因子受体对肝癌细胞增殖和转移的影响[J].介入放射学杂志,2021,30(01):43-47.
　YANG Peng,HE Yang,LI Cheng,et al.The effect of microRNA-7 regulating epidermal growth factor receptor on the proliferation and metastasis of hepatocellular carcinoma cells[J].journal interventional radiology,2021,30(01):43-47.
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微小核糖核酸-7调控表皮细胞生长因子受体对肝癌细胞增殖和转移的影响()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
30
期数:
2021年01
页码:
43-47
栏目:
实验研究
出版日期:
2021-01-25

文章信息/Info

Title:
The effect of microRNA-7 regulating epidermal growth factor receptor on the proliferation and metastasis of hepatocellular carcinoma cells
作者:
杨 鹏;  何 洋;  李 成;  王黎洲;  蒋天鹏;  许国辉;  周 石
Author(s):
YANG Peng;  HE Yang;  LI Cheng;  WANG Lizhou;  JIANG Tianpeng;  XU Guohui;  ZHOU Shi.
College of Medical Imaging， Guizhou Medical University，Guiyang， Guizhou Province 550004， China
关键词:
【关键词】 肝癌;  微小核糖核酸-7;  表皮细胞生长因子受体;  增殖;  迁移
文献标志码:
A
摘要:
【摘要】 目的 探讨微小核糖核酸（miR）-7是否通过靶向调控表皮细胞生长因子受体（EGFR）对人肝癌细胞侵袭和增殖产生影响。方法 传代培养HepG2人肝癌细胞，脂质体转染miR-7后使其表达上调或下调。荧光定量聚合酶链反应检测转染效率。蛋白印迹法检测miR-7上调或下调后肝癌细胞EGFR表达变化。溴化噻唑蓝四氮唑（MTT）法检测miR-7上调或下调后肝癌细胞增殖能力。划痕试验检测miR-7上调或下调后肝癌细胞迁移能力。结果 脂质体转染HepG2人肝癌细胞可控制miR-7表达，miR-7表达与肝癌细胞EGFR表达呈负相关。miR-7表达上调时，肝癌细胞增殖和迁移变慢；反之，miR-7表达下调时，肝癌细胞增殖和迁移变快。结论 miR-7靶向调节EGFR表达，其表达与肝癌细胞增殖和迁移呈负相关。miR-7可能是未来肝癌治疗新策略的潜在靶点。

参考文献/References:

［1］ 杨登科，黄 智，张 帅，等． miR-23b直接靶向IL-11抑制SMMC-7721细胞增殖研究［J］． 介入放射学杂志， 2019， 28：358-366．
［2］ Li B， Liu L， Li X， et al. miR-503 suppresses metastasis of hepa-tocellular carcinoma cell by targeting PRMT1［J］. Biochem Biophys Res Commun， 2015， 464： 982-987.
［3］ Li J， Fang L， Yu W， et al. MicroRNA-125b suppresses the migration and invasion of hepatocellular carcinoma cells by targeting transcriptional coactivator with PDZ- binding motif［J］. Oncol Lett， 2015， 9： 1971-1975.
［4］ Zheng C， Li J， Wang Q， et al. MicroRNA-195 functions as a tumor suppressor by inhibiting CBX4 in hepatocellular carcinoma［J］. Oncol Rep， 2015， 33： 1115-1122.
［5］ Hansen TB， Kjems J， Damgaard CK. Circular RNA and miR-7 in cancer［J］. Cancer Res， 2013， 73： 5609-5612.
［6］ Wang F， Qiang Y， Zhu L， et al. MicroRNA-7 downregulates the oncogene VDAC1 to influence hepatocellular carcinoma prolife-ration and metastasis［J］. Tumour Biol， 2016， 37： 10235-10246.
［7］ Normanno N， De Luca A， Bianco C， et al. Epidermal growth factor receptor（EGFR） signaling in cancer［J］. Gene， 2006， 366： 2-16.
［8］ Caratelli S， Arriga R， Sconocchia T， et al. In vitro elimination of epidermal growth factor receptor- overexpressing cancer cells by CD32A- chimeric receptor T cells in combination with cetuximab or panitumumab［J］. Int J Cancer， 2020， 146： 236-247.
［9］ 赵静怡． 癌症与非编码RNA（microRNA与IncRNA）关系网络的构建和分析［D］． 苏州： 苏州大学， 2017．
［10］ Song Y， He S， Zhuang J， et al. MicroRNA-601 serves as a potential tumor suppressor in hepatocellular carcinoma by directly targeting PIK3R3［J］. Mol Med Rep， 2019， 19： 2431-2439.
［11］ Yegin Z， Aydin O， Koc H， et al. Expression profiles of proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 in bladder cancer［J］. Cell Mol Biol， 2018， 64： 66-73.
［12］ Karakatsanis A， Papaconstantinou I， Gazouli M， et al. Expression of microRNAs， miR-21， miR-31， miR-122， miR-145， miR-146a， miR-200c， miR-221， miR-222， and miR-223 in patients with hepatocellular carcinoma or intrahepatic cholangiocarcinoma and its prognostic significance［J］. Mol Carcinog， 2013， 52： 297-303.
［13］ Choi KJ， Baik IH， Ye SK， et al. Molecular targeted therapy for hepatocellular carcinoma： present status and future directions［J］. Biol Pharm Bull， 2015， 38： 986- 991.
［14］ Xie J， Chen M， Zhou J， et al. miR-7 inhibits the invasion and metastasis of gastric cancer cells by suppressing epidermal growth factor receptor expression［J］. Oncol Rep， 2014， 31： 1715-1722.
［15］ Giles KM， Barker A， Zhang PM， et al. MicroRNA regulation of growth factor receptor signaling in human cancer cells［J］. Methods Mol Biol， 2011， 676： 147-163.
［16］ Cui YX， Bradbury R， Flamini V， et al. MicroRNA-7 suppresses the homing and migration potential of human endothelial cells to highly metastatic human breast cancer cells［J］. Br J Cancer， 2017， 117： 89-101.
［17］ Liu L， Yang X， Li NF， et al. Circ_0015756 promotes prolife-ration， invasion and migration by microRNA-7- dependent inhibition of FAK in hepatocellular carcinoma［J］. Cell Cycle， 2019， 18： 2939-2953.

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备注/Memo

备注/Memo:
（收稿日期：2020-02-08）
（本文编辑：秋 实）
更新日期/Last Update: 2021-01-25