[1]李腾飞,时程程,余 笋,等.巴马小型猪颈动脉粥样硬化性狭窄模型建立技术探讨[J].介入放射学杂志,2022,31(12):1179-1184.
 LI Tengfei,SHI Chengcheng,YU Sun,et al.Discussion on the model building technology of Bama mini-pig carotid artery atherosclerotic stenosis[J].journal interventional radiology,2022,31(12):1179-1184.
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巴马小型猪颈动脉粥样硬化性狭窄模型建立技术探讨()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
31
期数:
2022年12
页码:
1179-1184
栏目:
实验研究
出版日期:
2023-01-11

文章信息/Info

Title:
Discussion on the model building technology of Bama mini-pig carotid artery atherosclerotic stenosis
作者:
李腾飞 时程程 余 笋 龙树海 马 骥 李 臻 任建庄 韩新巍
Author(s):
LI Tengfei SHI Chengcheng YU Sun LONG Shuhai MA Ji LI Zhen REN Jianzhuang HAN Xinwei.
Department of Interventional Radiology, First Affiliated Hospital of Zhengzhou University & Interventional Institute of Zhengzhou University, Zhengzhou, Henan Province 450052, China
关键词:
【关键词】 动脉粥样硬化性狭窄 球囊扩张 巴马小型猪 颈动脉天冬酰胺内肽酶
文献标志码:
A
摘要:
【摘要】 目的 探讨血管内介入法构建猪颈动脉动脉粥样硬化(AS)狭窄模型的方法和要点,并观察天冬酰胺内肽酶(AEP)表达水平。方法 按照随机数字表法将24头巴马小型猪分成实验组(12头)和对照组(12头)。术前实验组给予高脂饲料喂养,8周末全身麻醉下经股动脉行颈动脉内大球囊过度扩张,构建颈动脉狭窄模型;对照组仅给予基础饲料喂养。入组时和造模后第1、8、16、24周末分别行体质量称量和血脂水平检验,采用彩色多普勒超声和DSA监测评估颈动脉狭窄形成。术后24周处死实验猪,收集狭窄处标本,苏木素伊红(HE)染色观察病理学改变,免疫组化染色分析AEP表达水平。总结血管内介入法构建猪颈动脉AS狭窄模型的技术操作要点。结果 高脂喂养后,实验组体质量和血脂水平均较对照组升高(均P<0.05)。术后24周,彩色多普勒超声和DSA证实实验组12头猪颈动脉AS狭窄模型构建成功,狭窄率为(33.21±13.17)%;与对照组相比,实验组狭窄组织病理学检查证实有明显的AS斑块形成。实验组颈动脉狭窄组织中AEP高表达,阳性细胞比率为(79.41±11.34)%,吸光度值为0.73±0.11,阳性评分12分;对照组中AEP低表达,阳性细胞比率为(27.13±17.34)%,吸光度值为0.41±0.14,阳性评分为6分,两组比较差异有统计学意义(均P<0.01)。结论 24周高脂饲养和大球囊过度扩张诱导颈动脉内膜损伤,可确切可靠地建立巴马小型猪颈动脉AS狭窄模型。AEP在巴马小型猪颈动脉AS狭窄病变中高表达,可能参与了AS斑块的形成。

参考文献/References:

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备注/Memo

备注/Memo:
(收稿日期:2022- 01- 27)
(本文编辑:边 佶)
更新日期/Last Update: 2023-01-11