[1]李 天,刘 冲,何金瞳,等.生物可降解镁对人胆管癌细胞和小鼠H22肿瘤抑制作用研究[J].介入放射学杂志,2021,30(08):792-798.
 LI Tian,LIU Chong,HE Jintong,et al.The inhibition effect of biodegradable magnesium on human bile duct carcinoma cells and mouse H22 tumors[J].journal interventional radiology,2021,30(08):792-798.
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生物可降解镁对人胆管癌细胞和小鼠H22肿瘤抑制作用研究()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
30
期数:
2021年08
页码:
792-798
栏目:
实验研究
出版日期:
2021-08-25

文章信息/Info

Title:
The inhibition effect of biodegradable magnesium on human bile duct carcinoma cells and mouse H22 tumors
作者:
李 天 刘 冲 何金瞳 张丹阳 隋凯达 张洲博 邵海波
Author(s):
LI Tian LIU Chong HE Jintong ZHANG Danyang SUI Kaida ZHANG Zhoubo SHAO Haibo.
Department of Interventional Radiology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province 110001, China
关键词:
【关键词】 可降解金属镁胆管癌小鼠H22肿瘤细胞凋亡
文献标志码:
A
摘要:
【摘要】 目的 评价可降解金属镁对人胆管癌细胞(RBE)和小鼠H22肿瘤的抑制作用。 方法 RBE细胞分别与纯镁和钛合金第1、3、5天浸提液共培养,细胞计数试剂盒(CCK)-8检测细胞毒性,流式细胞仪分析细胞凋亡。采用H22荷瘤小鼠模型,分别植入纯镁、钛合金后,于第3、6、9、12、15天测量肿瘤重量和体积变化,并通过钼靶X线摄影观察金属在体内降解情况。脱氧核糖核苷酸末端转移酶介导的缺口末端标记法(TUNEL)染色和免疫组化染色检测细胞凋亡和相关蛋白表达水平。结果 第1、3、5天,纯镁浸提液和钛合金浸提液对RBE细胞抑制率分别为24.7%、60.2%、70.7%和-3.1%、1.3%、1.0%,差异均有统计学意义(P<0.05);细胞凋亡率分别为13.8%、33.8%、46.7%和6.1%、7.1%、5.6%,差异均有统计学意义(P<0.05)。随着时间推移,纯镁在小鼠H22肿瘤中可部分降解。纯镁组肿瘤生长受到抑制,第9、12、15天平均体积(5.6 cm3、8.2 cm3、11.2 cm3)均显著低于钛合金组(11.4 cm3、18.0 cm3、21.7 cm3)(P<0.05)。纯镁组、钛合金组凋亡细胞比率分别为9.7%、5.3%(P<0.05),纯镁组小鼠肿瘤组织碳酸酐酶(CA)Ⅸ蛋白表达与钛合金组相比显著降低(P<0.05),其上游蛋白低氧诱导因子(HIF)-1α虽有下降,但差异无统计学意义(P>0.05)。 结论 可降解金属镁可抑制RBE细胞和小鼠H22肿瘤生长。

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备注/Memo

备注/Memo:
(收稿日期:2020-08-28)
(本文编辑:边 佶)
更新日期/Last Update: 2021-08-18