[1]嵇再雄,李家祺,王建波.酸枣仁皂苷B通过调节自噬抑制血小板衍生生长因子-BB诱导的血管平滑肌细胞增殖和迁移[J].介入放射学杂志,2021,30(02):149-152.
 JI Zaixiong,LI Jiaqi,WANG Jianbo..Jujuboside B inhibiting PDGF-BB-invoked proliferation and migration of vascular smooth muscle cells through regulating autophagy[J].journal interventional radiology,2021,30(02):149-152.
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酸枣仁皂苷B通过调节自噬抑制血小板衍生生长因子-BB诱导的血管平滑肌细胞增殖和迁移()

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《介入放射学杂志》[ISSN:1008-794X/CN:31-1796/R]

卷:
30
期数:
2021年02
页码:
149-152
栏目:
实验研究
出版日期:
2021-02-25

文章信息/Info

Title:
Jujuboside B inhibiting PDGF-BB-invoked proliferation and migration of vascular smooth muscle cells through regulating autophagy
作者:
嵇再雄 李家祺 王建波
Author(s):
JI Zaixiong LI Jiaqi WANG Jianbo.
Department of Interventional Radiology, Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai 200233, China
关键词:
【关键词】 酸枣仁皂苷B 血小板衍生生长因子-BB 血管平滑肌细胞 自噬 再狭窄
文献标志码:
A
摘要:
【摘要】 目的 探讨酸枣仁皂苷B(JuB)对血管介入术后血小板衍生生长因子(PDGF)-BB诱导的血管平滑肌细胞(VSMC)增殖和迁移的潜在影响及其可能机制。方法 25 ng/mL PDGF-BB刺激大鼠胸大动脉平滑肌细胞A7r5建立损伤模型。不同剂量JuB预处理后,用细胞计数试剂盒(CCK)-8检测A7r5活性,选出合适的用药浓度。实验分组为正常对照(NC)组、PDGF-BB组、JuB(10、25、50 μmol/L)+PDGF-BB组。免疫印迹法检测增殖细胞核抗原(PCNA)蛋白表达,划痕愈伤实验检测各组A7r5细胞迁移能力,免疫印迹法检测A7r5细胞自噬标记物微管相关蛋白1轻链3(MAP1LC3,LC3)B蛋白表达。结果 PDGF-BB处理后,A7r5细胞增殖和迁移能力显著上调(P<0.001),JuB呈浓度依赖性显著抑制PDGF-BB诱导的A7r5细胞增殖和迁移(P<0.001),JuB显著逆转PDGF-BB诱导的LC3B-Ⅱ蛋白表达水平(P<0.001)。结论 JuB可抑制VSMC异常增殖和迁移,机制上可能与其抑制PDGF-BB诱导的VSMC自噬有关,提示对血管介入术后新内膜增生引起的再狭窄具有治疗作用。

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备注/Memo

备注/Memo:
(收稿日期:2020- 09- 03)
(本文编辑:边 佶)
更新日期/Last Update: 2021-02-22